Slimeless cyanobacteria?

[FeltchWyzard]

Hey guys, I've been chasing an issue with my cloner where I'm just not getting roots and my cuts end up waterlogged like over cooked asparagus. There isn't any slime, it's like they have broken down cellular structure. Some have even just fallen off. I also noticed that my mix keeps increasing in pH. Could I have a bacteria that is causing issues but isn't sliming?

Here's some deets on my clone regimen.

I cleaned my cloner with h202 and let it dry completely for a day.
I cut long with a scalpel and put immediately in water.
Remove excess foliage, clip leaf ends.
I make the final cut 1/2" below a node under water at an angle, then use the blade to skin off a little stem skin above it.
I go immediately into clonex gel and into the collar with the jets spraying.

My mix is 10ml per liter of clonex solution and 2 drops of both roots xl and superthrive pHd to 5.8 and let stabilize for several hours at a room stabilized temperature.

I'm using a turbo kloner with humidity dome under t5's with a good bit of distance. Room temps in mid 70's

I've used other cleaning products for my cloner, like physan and even antibiotics, and still had little or no roots if my plants are infected could it then reinfect my cloner in a single run?

Anyone have similar issues? Anything anyone would add to my cloning technique?

I'm thinking of starting to use ogbiowar in my cloner but I'm afraid it might clog my jets.

Thanks in advance!

 

[amanda88]

I clone much the same in a diy bubble cloner, compressor only not the water jets,

I cut the same as above but use zero anything else I want them out and in soil in 10 days

my clones have lasted easy 10 days food free, and see all that junk above as a likely cause of bacteria,

I have used honey many times when I thought theirs gonna be a delay due to weather or what not

all you are doing is teasing out rootlets from under the bark, it ain't rocket science ...lol

good luck

 

[FeltchWyzard]

I've tried doing just water and clonex gel, tried just water on its own, too. I'm also not sure if it is a bacteria because I haven't found any slime, it's almost like the cuttings are getting cooked within a few days, but above the collars they are perky and green. I haven't heard of bacteria nasty enough to make cloning impossible without also leaving slime.

 

[Crusader Rabbit]

I had similar experiences with cloning and it turned out to be fusarium that was causing the problems.

https://www.icmag.com/ic/showthread.php?p=6766360#post6766360

 

[FeltchWyzard]

Crusader Rabbit I read your link and what you describe is just what I am experiencing. No discoloration on roots, but I do get woody and kinda purple stems that are no longer fleshy or hollow on my veg plants. Sometimes new shoots would come about usually as a result of repotting or heavy flushing both followed by beneficial inoculation but they seem to eventually get affected. My leaves show all kinds of issues(like they keep getting locked out even when runoff is under 200 and ph seems fine) but most prevalent are that they are tiny and limey. I don't get the brown total death that I see from examples of fusarium. It's more akin to a leprosy than black death necrosis. If plants survive to flower they seem to do fine but small buds and low yield. I'll post some pics tonight.

Did you succeed in ridding your plants of this malady? Are you sure it ended up being fusarium? Did you try ogbiowar or actinovate? I keep thinking I am getting ontop of this and it keeps escaping me.

 

[Avinash.miles]

water temps could be high? i run my cloner 15 minutes on and 15 minutes off cycles to keep res temps low (recommended by manufacturer & other peeps).... with just em1 / quantum lite & vsc in the res....

 

[FeltchWyzard]

My turbo kloner has a fan that pulls air through it, keeps cool. In the summer I have it 15on/off in addition but my temps are pretty low, in the high 70's.

 

[Crusader Rabbit]

I am finally having success by growing in large no-till containers of biologically active organic soil. Seeds were germinated in the containers to avoid exposing naked roots to external pathogens. The emphasis was in first establishing a healthy biologically active soil. I initially added beneficial micorrhizae to the mix, but did not use Rootshield because it is composed of Trichoderma which is a fungus that preys upon other fungi. I didn't want a single type of introduced predatory fungus to decimate the biological community needed to out compete the fusarium.

I recommend that all your cloning methods should emphasize isolation of cuttings from one another. Give up on the community water bath.

 

[FeltchWyzard]

I think at this point I'll be scrapping any notion of saving my strains and start new, don't want to risk it. How hard is this stuff as far as cleaning off your equipment? Should a good physan scrub and spray take care of things? Can this stuff go airborne or would it just be surfaces? Here are some pics of my worst vegging plants. I hope I don't get kicked from icmag for posting these!

 

[FeltchWyzard]

I started a new thread as I am now pretty certain this is more of a fungal issue. I cut open that woody plant and biopsied the stalk, found brown frown growth in there, posted pics in the new post.