sexing seedlings

[Miraculous Meds]

goal:

To sex plants at a small stage in solo cups to save space, reduce male population, reveg, clone, transplant, then flower.

I want to keep the sexing stage as short as possible, to have as little problems as possible with reveg'ing.

Do u have to force flower till u see sex declare, or can u flower for a week, put back into veg light cycle to start to reveg, and sex still declare?

 

[BAKED_BEANZ]

not a bad question...... looking forward to see this answer from someone with some rep. is also going to depend on sativa or indica? as sativa take longer to show sex?

 

[_tessarecting]

You're messing with the hormones by revegging and any speed gained is counterintuitively lost by this transition. If speed is what you are going for you want a strain that sexes fast and just let them go.

 

[iBogart]

I've never reveged a plant before, but I would just grow your seedlings until they're big enough to clone and sex one of the clones. Am I not getting it?

 

[_tessarecting]

Use smaller pots and or plant multiple beans per pot and cull as you go.

 

[burningfire]

I keep them in small pots.. I put the light far enough to get enough stretch so I can take cuttings and root them more easily.. after 2 nodes, I'll take the cutting, root it meanwhile I will flower the base.

 

[sourpuss]

Clone and flower prolly the best solution.... although thats the norm and I feel like u prolly wouldnt be asking this if that was easy for you.... personally id clone the tops... topping the plant... and flower out the topped plant not the clone... imo quickest way....

 

[_tessarecting]

Topping will cut 2 weeks from your potential flower date but is necessary if you want to supercrop.

 

[Miraculous Meds]

U guys missed my point. its not about speed. its about space. I don't want to transplant x number of seedlings for space reasons. so if I can sex them in solo's, it will save the space I want to dedicate to this project. Ill be able to cut the plants by half by pulling all the males. then I only half to transplant the females. And that amount of plants will fit in the grow space I have decided to use for this project.

So the main thing I want to know is, will the plants that haven't shown sex yet, that I bring back into veg after a week in flower eventually show sex. Or do I need to keep them in flower till they show. Im 7 days in and already culled one male, and am close to confirming several more. Id rather start the reveg process as soon as possible to limit the stress of revegging, and be able to flip them back into flower as soon as possible. that will be after I tp them, and get a clone, and they show positive vegetative re growth.

sorry for the confusion fellas, I had a hard time wording the first post so my question was clear.

 

[DrFever]

well what do you want????
rom seed 12 /12 top n take clones mark em solo cups ?? not sure might stress and possibly hermie plants maybe 1 - 2 gallon might be better
as for reveg
Well that will kill you expect 3 - 4 week for them to turn around after there sexed
or what i would do if i was in a rush i would veg them seeds normally take clones and place them in 12 / 12 marked which every way people tend to think there is a faster way well really i do not think there is your looking @ 4 - 6 weeks either way

clones taken from not a mature plant ??? will be a clone from a un mature plant it will still grow on 12 .12 until its mature enough to start to flower

 

[Crusader Rabbit]

If you're growing in coco you can keep them in beer cups under veg lighting until they show preflowers. I've done it in soil also, but they get root bound. Cull the males and transplant the females whole .... or chop them up to get clones, and short topped rooted females.

If you put a plant into 12/12 lighting for a week then back into 18/6 or 24, eventually it will show preflowers. But if you leave it in veg the whole time it will eventually show preflowers too. So why bother with the hassle of moving it to 12/12?

 

[Miraculous Meds]

well what do you want from seed 12 /12 top n take clones mark em solo cups ?? not sure might stress and possibly hermie plants maybe 1 - 2 gallon might be better
as for reveg
Well that will kill you expect 3 - 4 week for them to turn around

sexing in solos to fit them in the space. then culling males (well ill keep a few 10 or 15 for testing). Then ill be down to half the number of plants, and ill tp to 1 gal coir. if they hermy like this then piss on em, I will cull them.

as to the killing 3 to 4 weeks for reveg time, now ur getting to the heart of my question. can I pull out the stuff im sexing after 1 week even if they haven't shown yet, to keep the reveg time to a minimum. it is what it is, as far as time needed for positive reveg growth. but im hoping I can cut a clone off em and flip 2 weeks later if it works out. I know if I let em keep going in flower to sex its gonna take longer for them to get the flower hormones out.

this idea comes from bog responding to somebody to flower for 5 days and put them back in veg and they will show sex. I was just looking for confirmation from a member that might have tried similar.

 

[Miraculous Meds]

If you're growing in coco you can keep them in beer cups under veg lighting until they show preflowers. I've done it in soil also, but they get root bound. Cull the males and transplant the females whole .... or chop them up to get clones, and short topped rooted females.

If you put a plant into 12/12 lighting for a week then back into 18/6 or 24, eventually it will show preflowers. But if you leave it in veg the whole time it will eventually show preflowers too. So why bother with the hassle of moving it to 12/12?

ive grown 3'ers in solos. its not ideal though. and I am trying to keep them somewhat small so I don't want to wait for natural sexing to take place cause that might take what, 6 weeks plus? I have already planned on lighting and space to accommodate 1 gallon pots for flower. so im working the rest of the details to fit into this space/light plan.

 

[DrFever]

idunno man 1 week is to soon i think some may claim they flipped and sex showed in week but who knows how long they vegged ??
No something that recently happened to me was i topped some plants before sex and litterally next day i saw BALLS veg 2 - 4 weeks n top

 

[Miraculous Meds]

idunno man 1 week is to soon i think some may claim they flipped and sex showed in week but who knows how long they vegged ??
No something that recently happened to me was i topped some plants before sex and litterally next day i saw BALLS veg 2 - 4 weeks n top

huh that interesting, thanks. I would have thought that topping would slow down sexing.

well im 8 days in and have sexed about 10 males and 2 females. I hope the females catch up. lol think im gonna pull em and throw em back in veg and see how it goes. I got to get another set in to do the same sexing scheme. got to make room.

 

[DrFever]

whats up with so many different strains ?? one thing i learned long ago is i refuse to grow so many diff strains at once cause before you know it its a cluster fuck things get mixxed up
for me no more then 3 strains thats it i got 2 now going PCK x cherry pie and VG's blue berry will fire up one more strain this fall from the PCK line thinking purple train wreck x pck and find a good pheno out of them

 

[Miraculous Meds]

whats up with so many different strains ?? one thing i learned long ago is i refuse to grow so many diff strains at once cause before you know it its a cluster fuck things get mixxed up
for me no more then 3 strains thats it i got 2 now going PCK x cherry pie and VG's blue berry will fire up one more strain this fall from the PCK line thinking purple train wreck x pck and find a good pheno out of them

Its a sickness. I cant help it. seriously though I know the advantages of running only one or 2 strains. I would chose that if getting yield was my main objective. but I love variety and so do my friends. so strain whore I am.

and I been reading up on breeding and got the bug. bad!!!

 

[Only Ornamental]

You could do a PCR, ELISA, southern/western blot, or similar analyses. There are over half a dozen recent publications on that subject. Established tests cost at a rough estimate about $ 5 per gene/protein (multiplexing would be cheaper but you don't need that for sexing), use very small sample amounts, and are often semi-automated and pretty fast and/or run several samples at once .
Alternatively, do karyotyping yourself. Best would be with a tiny root tip, some chapo DNA dye, a good microscope, and some luck to find a cell wherein you can distinguish the X and Y chromosomes.
In my limited experience, the more vigorous seedlings and faster growing young plants tend to be males but this is, like any other visual characteristic, not reliable at all (and very inconvenient as one tends to keep the healthier plants which would, in this case, be males LoL).

 

[sourpuss]

air layering.... might help speed up yhe cloning process...

 

[C. Breeze]

Why can't you just leave them all in 12/12, cull the males and cut clones from the females after they sex? When I pop a bunch of new beans, I usually veg 2-4 weeks, depending what else is going on, and then throw them all into flower. Males get separated and collected or discarded - and as the females sex I cut a clone or two from each to root- leaving the seed plant in 12/12 to finish. The clones still root in perlite and are ready for soil in 10-11 days. If the plants are stretching hard I usually wait until that slows- but before hard bud set to cut clones. I've cut clones while girls were stretching there nodes a lot- and come in the next morning to clones that were 1/2-1" taller- and pale green.... Overnight. Still got 50-100% rooting success- but they weren't ready to rock in 14 days.