refridgerate for purpling?

[A+Dank]

hi,
I am wondering about purple buds ...
i had this thought that for those of us who dont have cold nights indoors and we want the purpling effect that some strains can give, how would putting a plant (pot and all) into a refridgerator at lights out for a while before the harvest? This would simulate cold nights, and is doable for the indoor grower in any climate, any time of year.
I dont know much about purple strains, so i dont know how many nights or how long before harvest one would want to do this cold treatment. Also, i guess that the fridge should not be on the coldest setting, cuz that could hurt your ladies, the target temp its like 50-55 for purpling, right?
I know alot of purpling is strain and pheno dependent, as opposed to being only the cold temps in the environment, but i would love to know if anyone has experience with this fridge idea or could at least help me to understand the purpling. Is it anthocyanin that turns it purple? how very curious, is this pheno in most cannabis, or just some landraces and/or hand select strains???...
I would also worry about getting moldy soil from some loose spore in the fridge, so the fridge is gonna be sanitized before i do this, but i am about 6 days away from harvest and thought, "hell maybe i can purple up a couple of these ladies...."
What do you all think? Could it work? is it a BAD idea for some reason i am missing? how many nights at low temp might be needed to get purple? what temps are safe, and what genetics typically show cold induced purple ? this is a lot of q's, but I figure that the people here are more qualified than anywhere else to ask. Thanks!

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p.s.
and...while im at it... is the indoor soil thread the best place for this? i am talking about indoor soil grow, lil 1.5' plants ...but this thing i am talking about it like some advanced technique...is there a board on these forums for fringe cannabis information and techniques, like sex determination by pcr or wierd light cycle experiment (18/6 and 12/12 from seed...).

 

[Verite]

When I feel like having colored bud I simply put on a pair of 3-D glasses.

 

[guineapig]

yes i see your logic there....honestly i can't remember seeing any experiments done on this subject...

so i guess we want to induce the production of anthocyanin pigmentation....
most crops have a purpling trait bred into them via genetic selection or have moved their greenhouses to the climates which naturally produce purple pigmentation, say like the highlands of Colombia (rose greenhouse facilities)....

the thing you wouldn't want to happen was a change in the delicate ecosystem which
you have created in your root zone.....if you are a rhizophile you have taken the time to maintain a healthy and balanced ecosystem which you wouldn't want to destroy.....however if you use an inert rockwool substrate which maintains a healthy plant that would be the best situation imho....

another concept lurking around here is the D.I.F. or the temperature difference between the roots and the shoots.....so you could somehow divide the environments of roots and shoots and then adjust each individual zone's temperature accordingly...

For more about the DIF please refer to my new book "GP's Guide to Gettin' Pounds"....

-kind regards from your humble guineapig

 

[A+Dank]

word
i am a rhizophile, and thogh its a soiless mix, there is a load of worm cast in there, so i know its alive. i didnt add any microrhizae cuz that is expensive and i havnt figured out how to crop some and culture it from somewhere else...so, yes, i would not want to shock my plants root zone.
im curious tho still about the temperature range that does induce the anthocyanin pigmentation. we have had some cool nights here, so i think the roots can take the fridge treatment. i might give this a go on the next round since i am days away from harvest. the cold will slow down the metabolism a whole lot, so much that i dont know if the pigmentation would occur in time. alas alack, there is always the next time.
anyone with any experience or further ideas about fridging some nugs, please chime in!
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[jaykush]

theres other ways of getting purple nugs, a simple drop of the ph to around 5.5 for a week should give some colors. how far into flowering are you? how long do youe expect left?

 

[Guest]

You are right in thinking you need both the genotype and phenotype to get purple buds but why would you want purple buds, they are no more potent than green buds.

http://www.mellowgold.com/grow/mjbotany-removed/marijuanabotany3.html

So far we have discussed inheritance of traits con trolled by discrete pairs of unrelated genes. Gene inter action is the control of a trait by two or more gene pairs. In this case genotype ratios will remain the same but phenotype ratios may be altered. Consider a hypothetical example where 2 dominant gene pairs Pp and Cc control late-season anthocyanin pigmentation (purple color) in Cannabis. If P is present alone, only the leaves of the plant (under the proper environmental stimulus) will exhibit accumulated anthocyanin pigment and turn a purple color. If C is present alone, the plant will remain green through out its life cycle despite environmental conditions. If both are present, however, the calyxes of the plant will also exhibit accumulated anthocyanin and turn purple as the leaves do. Let us assume for now that this may be a desirable trait in Cannabis flowers. What breeding techniques can be used to produce this trait?

 

[Guest]

You are right in thinking you need both the genotype and phenotype to get purple buds but why would you want purple buds, they are no more potent than green buds.

Bag apeal?

 

[A+Dank]

hey,
yea, im growing for my own head, but like to mess around once i start a project, push the limits, that is why i would like to see if i can induce purpling. I have chopped half the crop, and am gonna chop the rest in the next few days, im checking out how the high will be different if they run a lil bit longer.
i am doing a soilless grow... what is a good way to drop the ph to 5.5 ish? orange juice? soda water? all that c02 should acid things up for a lil bit. i have no ph meter or way to take soil sample, mix with water and use litmus paper...its too inaccurate, i cant get a ph reading cux i dont know how, but....
one thing i am not sure about is: in purpling strains, how long does it take for the pigmentation to form? i am thinking, more than days...but on seedlings, the anthocyanin is present b4 clorophyl on their lil stems when they are stretching fast...
Basaedo...the genetic linkage you are talking about...its this been traced using forward genetics? are these alleles for purpling, or is this speculative? wouldnt dominant C and P give rise to wt green plants?
Here is the best i can do... I am going to throw one of my ladies in the sanitized mini fridge set on 4 (second to lowest setting, reading 47-50 on the thermometer) for the last 48 hours of dark. if there is a difference in this plant versus the others, if i can get a digi cam, i will post the results.
I have no idea about the mechanism of biosynthesis for anthomyacin, or if it is expressed in the leaves/calyx, so i dont know if it will happen in the dark, but i guess the cold turns stuff purple, as well as the ph and sometimes raw genetics (ne one see lightstorm (skywalker x sand storm) by gotpurple bud on OG? it was MAD purple calyx, through the whole bud, not just the superficial cells, and it was not temp or ph induced, it was the genetic pheno type).
jaykush: I will try a mix of OJ and soda water in the cold box and see what happens, i only have a few days left before the chop.
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p.s. oj is not some fool move to try to make orange flavored purple bud! its for the acid yo!

 

[Guest]

ermm, A+...

Consider a hypothetical example where 2 dominant gene pairs Pp and Cc control late-season anthocyanin pigmentation (purple color) in Cannabis.

Thats what it said.


Turkish wrote

Bag apeal?

Oh, like this you mean... ?

 

[Guest]

Nah this ones more my style

 

[A+Dank]

ooo. nice purple bags... and look at all the purple hairs! egads, i think i would pay 150 dollars a gram for weed that looked like that, and then i would put it in my flying ganja museum.
Baseado... I dig, i follow you are talking about two different alleles, P, dominant for purple leaves, C dominant for green, but the two complement to make purple on the whole plant. The trick is first to find a purple strain, cross it and then back cross it and screen all of your F1's for any purple traight, then start back crossing those f1's to the purple parent or do sibling crosses between the purple F1's... i guess you could hermie your purple plant and self it, that might save time in the long run too, but honestly, i dont know if that would work, that is just what i would try. im no breeder tho. Tonite is night one in the fridge for my most immature bud (most clear trichs and most undeveloped calyx, least nute loss in the fan leaves (cuz they are DAYS away from flower. the ones that got the chop already are starting to stink up worse than when they grew! holy purple cows! ganja abounds!
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[A+Dank]

Sucess!
sorry...no pics.... NEXT TIME...b/c pics is what eddyone wants...
ok, well, the fridge treatment got me some verrrry blue/purple hues in the leaves. There was some nute deficeincy starting in the bottom fan leaves, serious N dep and looking like some other wierd spotting i figure just because the plant is giving everything to its buds...but that is different from the purpling in the leaves up in the colas. No calyx is changing...but definetly some serious blueing purpling up in the leaves of the nugs commpared to the room temp buds. hmm hmm cold room treatment... definetly gonna run that AC in the summer if its still indoors.... sorry everyone to be a tease w/o no pics. i will try to borrow a digi cam and get some photos up. peace and kindness, thanks for all the good talk about anthcyanin, i have no idea what flavinoids are being expressed in this bud, but im gonna send em last couple into reveg. cuz i like em alot!
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[jaykush]

A+dank glad to hear the fridge did somewhat of a coloring to your plants, youll need to put them in there for at least a week to start changing calyx colors as well, the leaves are nothing really there going to be trimmed off anyways.

 

[foxygrower]

I have seen an application of Clearex or a similar flushing agent bring out some nice colors in the leaves. In term of purple Calyxes..... I think thats only phenotype dependent. I have seen plants with Green leaves that have purple buds and plants with purple leaves and green buds.... and plants with both purple leaves and purple buds....................... what are you looking for . I'd say Purple buds with green leaves would look the coolest. Thats what im hoping with my Double Purple Doja.

Peace,
FG