Pollen Watersoaking for precise Otdoorpollination ? Possible?

[romanoweed]

I heard there is the possibility of moisturize Pollen to pollinate a single Branch??

Just soak Pollen in Water short before going to your Plants, and the Pollen doesent die in this short Period, and rather savely pollinates single Branch.
Without flying far.

Is that true?

What about other Liquids such as liquid Air, liquid oxygen.

[FONT=Arial, Helvetica, sans-serif]https://en.wikipedia.org/wiki/Liquid_oxygen[/FONT]

 

["Username"]

Wet pollen will definitely not work! Water prevents germination, once the pollen gets wet it won't be viable and won't make seeds.

This is what I do when lightly seeding plants in the bush, I dab my finger in the pollenI collect (or use a small artist's brush) and gently sprinkle or paint a small amount of the pale yellow powder on the buds you want seeded. A little goes a long way. So long as it's not a windy day the pollination will occur where you want it to and not everywhere.

You can also grow males in the same plot as females and if you get your timing right, you will control which buds get pollinated. Do this by cutting back the breeding males to one or two lower branches and remove the tips when they first show sex. Then you have to watch them, remove any early flowers that will drop pollen earlier than you want, sometimes you have to cut back most of the male buds to control when you want pollination to happen.

Cutting the soon to be maturing male flowers will retard the pollen drop by a couple of weeks which will also mean the females will be bigger. If you (even lightly) pollinate small females when they first form little tip buds then your yield could be reduced, so it's best to wait a few weeks then hit them with the pollen.

Also, you really want to pollinate so the seeds have time to completely form before harvest and also not be fully formed a month before harvest either or some might fall out of the seed bracts and onto the ground. It depends on a number of factors, but you should get good viable seed in 6+ weeks on healthy plants in full sun, this is why you pollinate lower branches so the sensi bud can be harvested when ready and the plant with the seeded branch can be left there for a few more weeks or more if required.

Timing is where it's at when growing males and females side by side in the bush and this takes experience. I like to remove the stems with male flowers a day or less away from them dropping pollen. Male flower that are puffy and yellow can be gently prised opened and they will release some pollen. Sometimes I just break off a male branch and dab a female branch with it, sometimes I take the branch home, keeping the cut stem moist and suspend it over a sheet of paper and catch the pollen and use it same or next day. Other times I just cut down the male and squeeze open a few almost ready male flowers and sprinkle that small amount of pollen on a little bud or two and end up with 20 or 30 seeds, a little goes a long way.Just one lower branch that is lightly seeded will produce 100's ~ 1000's of seeds.

Happy seed making!

 

[romanoweed]

Yes thanks for this Description, but i heard this Watermethod workde for someone, and im wondering if you know for a Fact it wont work, or if you assume it.

I am aware that Water is used to disable Pollen from doing their Job, i know i know.
But consider, binding pollen would be perfect Thing.
I search perfect save Solutions for Breeding

 

[grayeyes]

Water kills pollen. Point blank. A time honored practice by growers is once you have pollinated the branches you want then cover them with a plastic bag and spray down the plant. Water kills the loose pollen.

 

[romanoweed]

But i heard it works

 

[romanoweed]

it works with Pinus, and Palms:

- https://www.fao.org/3/v3030e/V3030E02.htm
- https://www.researchgate.net/public...ion_and_facilitate_date_palm_field_operations

Also they colored Pollen to see if its precice, and it was

 

[Cvh]

Water makes the pollen sprout/germinate, hence killing it.

Google a bit to learn how pollen works and how it creates the pollen tube to reach the female ovule.
(Water makes the pollen prematurely creates it pollen tube aka sprouting/germinating.)

 

[Cvh]

 

[Cvh]

Watch this video.
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[romanoweed]

Thanks very much Chv. From what i understand: moisture is Causing that Pollen to come (ejaculate) before it reached the Stigma.

I googled for moistureless Liquids and found per Example Alcohol can be 99,9 percent pure. Probably that would work?? It doesent contain Moisture if i understand right. And Oils? (Alcohol might be to harsh for Sperm)

And it is also unclear if it still would work with water, some Pollensperms might still randomly make it .

 

[Wallynoland]

Here's something that may be useful that Nevil forwarded to me.

Hi Nevil,
we spoke once or twice over Skype—I am the guy with the high-tech food growing publication that works with Alfie at times. Not sure if you would remember the talk. Anyways, I am researching the notion (or maybe it’s done all the time already) of using Fem pollen held in liquid suspension. Do u know of this as an established process? i.e. screen pollen, add to sugar water and spray. Seems way more efficient and might allow the use of several pollen sources in the same room (i.e. more control in the spray versus dust). I found this paper from Washington State University that suggests increases in fruit sets like this (apples, cherries, etc). http://jenny.tfrec.wsu.edu/wtfrc/PDFfinalReports/2016FinalReports/WhitingPollinationFinal.pdf
Realize you prob can command a good buck for your knowledge—hoping you might “donate” a quick thought on it? The paper is good stuff, IMO. I could send a saved PDF if u don’t want to click the link or whatever. Thanks for consideration, and an eternal fan here,
2. Suspend pollen in liquid
In 2014 we conducted lab studies on pollen viability and the role of suspension media components. From our tests of three sweet cherry pollen genotypes (Lapins, Rainier, Sweetheart), we found that we could improve pollen viability (i.e., germination) when pollen was suspended in a modified medium, compared to water. For each genotype, the following suspension mediums were evaluated: solution 1: 5% (w/v) sucrose + 0.15% (w/v) pollen grain, solution 2: 10% sucrose (w/v) + 0.15% (w/v) pollen grain, solution 3: 15% (w/v) sucrose + 0.15 % (w/v) pollen. Pollen germination declined over time in the control treatment with only water. This occurred for all cultivars similarly, losing about 20% germination from initial suspension to 60 minutes later. In contrast, the suspension solutions improved pollen germination and extended viability over time. There appears to be a benefit to higher rates of sucrose, with viability improved more than 600% after one hour in suspension. Suspension medium containing 15% sucrose (w/v) improved germination significantly in all pollen genotype irrespective of how long the pollen was in suspension. This is likely due to the favorable osmotic balance in the medium with sucrose. In contrast, we documented approximately a 15-20% decrease in the viability of pollen grains in control between the first and last sampling time. The highest pollen germination was observed with Sweetheart (67%) followed by Rainier (59%) after 40 minute suspension time in media enriched with 15% sucrose (w/v).
In 2015, pollen suspension development continued with multiple experiments carried out to investigate the role of candidate suspension components on pollen viability. For our in vitro pollen germination experiments, we utilized one sweet cherry, apple and pear genotype (Rainier, Red Delicious and Bartlett). Cultivar selections were based on most suitable and compatible pollen genotypes to source pollen for our field trial pollen receiving cultivars. In every case, pure pollen was utilized. Candidate suspension components were selected from a literature review of relevant scholarly articles. Initially we evaluated pollen viability after incubation of 1 hour at a concentration gradient of each the components. Afterwards, we assessed combinations of components on pollen viability. In each case we used 0.18% w/v of pollen for the experiments. Replicate tubes of suspension materials were created, pollen was added, and the tubes were agitated briefly (<5 sec) before incubated at room temperature. Suspension aliquots were withdrawn after 5, 30 , and 60 minutes and pollen germination was evaluated to assess viability. Following suspension, our modified medium maintained or significantly improved pollen germination percentage compared to only water, irrespective of pollen genotypes and cultivars. Recent findings revealed that, using sucrose at lower rate could maintain favorable osmotic balance retention with optimal pollen
viability, either alone or in combination of other components in suspension, across all three genotypes.
60
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a
a
a

b

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00 Control Suspension Suspension
media + media + Ad- Regulaid Wet 90
Control Suspension Suspension Suspension media w/o media + media + Ad
surfactant Regualid Wet 90

Figure 1: Effects of suspension media on sweet cherry (left) and apple (right) in vitro pollen germination after 60 minutes in suspension (both p-values: 0.000).
Pollen + water alone is not an effective combination. First, pollen is not easily wettable (i.e., difficult to suspend); secondly, pollen losses viability in water. Germination rate of pollen suspended in water consistently decreased over the incubation periods, and reached a minimum after 60 minutes. We evaluated candidate suspension components individually and in combination for their effect on pollen germination. After 5 minutes in suspension with water + a single suspension ingredient, several suspension components improved germination – rates were 151% and 144% of control for ‘Red Delicious’ pollen. Similar results were found with cherry and pear pollen. We then combined suspension constituents at their optimum concentration and found improved pollen germination (206% of control). After 30 minutes of pollen in suspension, we found great improvements in pollen germination. Several suspension components increased germination by 220% to 230% of control. The greatest improvements in pollen viability were seen after 60 minutes of pollen incubation, when our suspension improved pollen germination rates that were more than three-fold greater that the control of water + pollen. Our combined suspension media improve pollen germination by more than 4x compared to the control after 60 minutes (Figure 1).
chart got jumbled when I copied and pasted. I won’t keep bugging you, lol. Would really value any thoughts you might have on this, ‘Ta

 

[Amynamous]

Anyone know how much time it takes for the pollen tube to grow and do it’s thing and fertilize the female egg?

 

[Cvh]

Anyone know how much time it takes for the pollen tube to grow and do it’s thing and fertilize the female egg?

Depends of the type of plant. I don't know for Cannabis/Hemp.

 

[djonkoman]

I googled for moistureless Liquids and found per Example Alcohol can be 99,9 percent pure. Probably that would work?? It doesent contain Moisture if i understand right. And Oils? (Alcohol might be to harsh for Sperm)

I think 99,9% alcohol might dissolve/disintegrate the cell membrane(=killing the pollen), unless that exine layer on the outside(looking at those nice pictures Cvh posted) can withstand and block the alcohol.

 

[romanoweed]

YES WALLYNOLAND!

Thats what i wanna hear!
Thanks for the Good Info and a propper Term for it: liquid Suspension

here they tell the same its patented: https://patents.google.com/patent/CN101617619A/en
just for other Species like Apple Trees, but who knows possibly for Cannabis too. Just need bit Sugar and viability goes up, huh.?
Consider they use this to make pollen longer storable, confusing how controverse..

 

["Username"]

Anyone know how much time it takes for the pollen tube to grow and do it’s thing and fertilize the female egg?

A pollinated female cannabis flower will show slight swelling (the formation of the seed) after several days that gets more obvious after each day .

If you want to store male cannabis pollen, try this proven easy method. Collect the freshly dropped pollen on a sheet of white paper, fold the paper in half several times, place it in a zip lock bag and put it in the freezer. Remove the package from the freezer when you want to make seeds, let it sit there on the counter until it is room temperature (or condensation will kill the pollen) then open it and sprinkle or paint the pollen on fresh female flowers.

 

["Username"]

The male pollen can be stored with dry white rice which will help keep it dry and rice is easily separated from the pollen when you want to use it.

 

["Username"]

Smokers tell non-smokers that they are missing out by not enjoying cannabis.

Growers tell smokers that they are missing out on the pleasure and satisfaction that comes from growing your own cannabis.

Seed makers tell growers they are missing out on the wonders of breeding cannabis.

Each step you take from smoker to grower to seed maker is a step up in enjoying cannabis to it's fullest! Maker that step and widen your enjoyment!

 

[zif]

Thought of this thread when I saw an article on using soap bubbles to improve hand pollination.

You can find the article here: doi:10.1016/j.isci.2020.101188

It has a number of references to work on liquid pollination techniques, too.

Let us know if you get it working!

 

[SUPER_HAZE]

In the cannabis cafe forum there is a post of several pages with very good information where several users tried it. For some, liquid pollination worked.