Abstract
Effect of different photosynthetic photon flux densities (0, 500, 1000, 1500 and 2000 μmol m−2s−1), temperatures (20, 25, 30, 35 and 40 °C) and CO2 concentrations (250, 350, 450, 550, 650 and 750 μmol mol−1) on gas and water vapour exchange characteristics of Cannabis sativa L. were studied to determine the suitable and efficient environmental conditions for its indoor mass cultivation for pharmaceutical uses. The rate of photosynthesis (PN) and water use efficiency (WUE) of Cannabis sativa increased with photosynthetic photon flux densities (PPFD) at the lower temperatures (20–25 °C). At 30 °C, PN and WUE increased only up to 1500 μmol m−2s−1 PPFD and decreased at higher light levels. The maximum rate of photosynthesis (PN max) was observed at 30 °C and under 1500 μmol m−2s−1 PPFD. The rate of transpiration (E) responded positively to increased PPFD and temperature up to the highest levels tested (2000 μmol m−2s−1 and 40 °C). Similar to E, leaf stomatal conductance (gs) also increased with PPFD irrespective of temperature. However, gs increased with temperature up to 30 °C only. Temperature above 30 °C had an adverse effect on gs in this species. Overall, high temperature and high PPFD showed an adverse effect on PN and WUE. A continuous decrease in intercellular CO2 concentration (Ci) and therefore, in the ratio of intercellular CO2 to ambient CO2 concentration (Ci/Ca) was observed with the increase in temperature and PPFD. However, the decrease was less pronounced at light intensities above 1500 μmol m−2s−1. In view of these results, temperature and light optima for photosynthesis was concluded to be at 25–30 °C and ∼1500 μmol m−2s−1 respectively. Furthermore, plants were also exposed to different concentrations of CO2 (250, 350, 450, 550, 650 and 750 μmol mol−1) under optimum PPFD and temperature conditions to assess their photosynthetic response. Rate of photosynthesis, WUE and Ci decreased by 50 %, 53 % and 10 % respectively, and Ci/Ca, E and gs increased by 25 %, 7 % and 3 % respectively when measurements were made at 250 μmol mol-1 as compared to ambient CO2 (350 μmol mol−1) level. Elevated CO2 concentration (750 μmol mol−1) suppressed E and gs ∼ 29% and 42% respectively, and stimulated PN, WUE and Ci by 50 %, 111 % and 115 % respectively as compared to ambient CO2 concentration. The study reveals that this species can be efficiently cultivated in the range of 25 to 30 °C and ∼1500 μmol m−2s−1 PPFD. Furthermore, higher PN, WUE and nearly constant Ci/Ca ratio under elevated CO2 concentrations in C. sativa, reflects its potential for better survival, growth and productivity in drier and CO2 rich environment.
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At 20 and 25 oC, WUE (water use efficiency) increased with increase in PPFD up to 2000 μmol m-2s-1 (Fig. 5). On the other hand, WUE increased only up to 1500 μmol m-2s-1 PPFD at 30 oC and decreased thereafter at higher light levels. Temperature higher than 30 oC had an adverse effect on WUE of this species. The maximum WUE was observed at 30 oC and under 1500 μmol m-2s-1 PPFD. Photosynthesis appears to have a greater influence than E (transpiration) over regulating water use efficiency in C. sativa. A highly significant positive correlation was observed between WUE and PN (r = 0.92). Together, high temperature and high PPFD had an adverse effect on the WUE in C. sativa.
But the fact you grow with LEDs mean the Pn will be low anyway
GL
Why do people always respond with stupid opinions that dont matter. This thread has nothing to do with HID. It seems they do prefer a little higher temp range, i let my get up to 85 and they seemed to love it but im running aero and didnt want my res temps going uo with it. Next run will be hempy and plan on letting it get around 85. Even with my HID plants performed fine in 85.
you'll need to back up that dubious assertion.
A common misunderstanding is by lamp makers who report irridiance by PAR, by people in cannabis world, and by most all grow shops and cannabis gurus that define PAR as a measure of irridiance, and it's not, it's a identification ("qualification") of photons which drive photosynthesis, that's all, and it is a range from 400-700 nanometers (wavelengths). Now, PPFD (Photosynthetic Photons Flux Density) is what the lamp companies mean when they write PAR, and PPFD measures ("quantitates") the irridiance of photons (umol) within a predefined area at the canopy within PAR range (400-700 nm); basically counting how many photons within PAR strike the canopy in a sq meter per second. That is the light measure to use for plants, NOT lumens, lux, Kelvin, footcandles, etc. To get more exact the PPFD per nanometer (not the total PPFD) needs to be weighed with the relative amount of photosynthesis provided from each photon in each nanometers, i.e., "Quantum Yeild", QY.
Cannabis has ideal-maximum rate of photosynthesis (Pn) with irridiance of 1,300-1,500 PPFD. Much over 1,500 PPFD and "photoinhibition" will lower Pn and Pnnet.
HTH
Thanks everyone! great points but yes I would like to hear more about this Pn shit.
I KINDA wanted to add UV to my garden, LED is a bit short of UV, heard this could help. has anyone done this? Do you know the spectrum range to look for, perhaps a place online to buy them?
I know adding like 2 cfl bulbs will add about 10 degress to my cab... Always could throw another LED unit in there as well. the 126w Penetrator adds about 10 degreees.
It's more about PAR than lumens. non-PAR lumens do not influence Pn.
You need to back up your dubious assertion.
http://www.icmag.com/ic/showpost.php?p=2869081&postcount=7
Looks like 1500w of LED can do plenty of intensity to be more than "inches" from the canopy... Perhaps you should be slower to jump to conclusions.
anyhow... I'll be setting up a proper 1000w hps vs. 504w LED test soon...
Should give me all the proof I need one way or the other.
I am one of a group that has been successfully growing with LEDs for the last couple of years. We don't sell our lamps, but all our work is public domain and everyone is free to go out and build their own.
M.P.
PAR is for plants.