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Finally a way to make pure Sativas flower in Summer!!

romanoweed

Well-known member
Florigen - Scientists have proven the existence of this flowering-inducing hormone or hormone-like molecule linked to the process of photoperiodism, although despite decades of research its mechanism is still a mystery. It was shown that grafting a leaf taken from a flowering plant onto a non-flowering plant was enough to induce flowering in the latter despite being kept in a non-flowering photoperiod. The same leaf can be removed and grafted onto another plant, giving the same results. Efforts to isolate and identify this molecule are ongoing, but it could theoretically be of great use to cannabis growers, potentially enabling photoperiod-independent flowering in non-auto genetics.
 

romanoweed

Well-known member
just another Exerpt form an Cannabis Article:

Maximum Yield Explains Florigen​

Florigen was first discovered in 1865 by German scientist Julius von Sachs.

Florigen production is triggered in short-day plants such as cannabis when the days start to shorten and the plant is exposed to longer periods of darkness. It has been discovered that exposing a marijuana plant to pure darkness for a period of 24 to 30 hours will stimulate its production of florigen and trigger its flowering cycle. This allows a grower to synchronize a cannabis plant’s pre-flowering stage and control its production by inducing flowering.

Scientists have also used florigen to grow tropical plants in hostile growing environments such as Northern Europe, a feat that was previously deemed impossible.
 

romanoweed

Well-known member
Another excerpt (the original article is closed but a copy exists with no pics..) :

This is about How to harvest Neviles Haze or other super long flowering Sativas in early September. Many many months ago while reading about flowering I happened upon a Mikhail Chailakhyan paper. He was the one that coined the term Florigen.
http://en.wikipedia.org/wiki/Florigen
I was working with grafting at the time and when I read his paper it floored me. The paper concerns longer flowering tobacco being flowered early by grafting with an early tobacco. The early tobacco was similar to autoflowers in weed. BINGO. Large lights flash in head. So I immediately plant some Tom Hills haze. My plan, graft a autoflower to a cloned THH and see if it flowers early. This rocked on for quite a while. My clones kept getting root rot and dying. The third set I got some to take. After letting them root good I grafted a NYLD auto to the THH with an approach graft. Basically skin away both plants and then tape together. Pics.
The small one on the top is THH.
105.jpg


close up
106.jpg


I was going to keep this to myself and do some breeding with Hazes. The decrease in flowering time would be a great boon to breeding. Unfortunately a tornado came, cops got everything, busted. Since I'm certainly not going to be growing any time soon I figured I'd tell everyone.

After I had started this I found someone in Russia was doing the same thing and had been successful with an autoflower grafted to a Kali Mist. "In Russia". You know they don't have the weather for Kali Mist there but he made it happen.

It's interesting but known that certain new technologys people seem to start doing them at the same time. Very odd but it happens quite frequently. I don't claim to be the first just that I had the same notion.

I hope someone will try this this year. Here's the results I know so far. The auto does not have to be as big as the 12/12 plant. It needs to be after the solstice before the 12/12 is grafted to the autoflower. It would be great to have a Nevilles Haze finish in Canada.
 

romanoweed

Well-known member
I wanna distance myselve a bit from the "third post, the guy who knows a russian who supposedly made it happen".

So, the first article does graft the Leaves of a flowering plant. And the russian guy i guess grafted the Rootstock of a flowering plant ---

------

So the initial Leaves-grafting convinced me the most and made me go wow!
Its also cause i found a Study lately that found out that Cannabis detects the Daylenght somewhere in the Axis (in lack of better memory i call it Axis) . It was such a hard Term to remeber, but i am firm it was somewher were the branches split in differnt directions, just the Axis, OR it was where the Leaves branch away from the Stems. One of the other.
But they said that cannbis detects DEFINITLY the light Cycles at those Axis. (so stupid i was too overworked to Bookmark that study)

And now that i read they used Leaves and its attached twig to induce flowering, it would be such a perfect Match of that Study with what the Article says about Florigen Hormones .
 

Mudballs2.0

Active member
I wanna distance myselve a bit from the "third post, the guy who knows a russian who supposedly made it happen".

So, the first article does graft the Leaves of a flowering plant. And the russian guy i guess grafted the Rootstock of a flowering plant ---

------

So the initial Leaves-grafting convinced me the most and made me go wow!
Its also cause i found a Study lately that found out that Cannabis detects the Daylenght somewhere in the Axis (in lack of better memory i call it Axis) . It was such a hard Term to remeber, but i am firm it was somewher were the branches split in differnt directions, just the Axis, OR it was where the Leaves branch away from the Stems. One of the other.
But they said that cannbis detects DEFINITLY the light Cycles at those Axis. (so stupid i was too overworked to Bookmark that study)

And now that i read they used Leaves and its attached twig to induce flowering, it would be such a perfect Match of that Study with what the Article says about Florigen Hormones .
You mean axial system i believe. I dont have a plant in veg just now big enough to graft onto or i would try it. Ive grafted cannabis before.
 

romanoweed

Well-known member
BUT i have only limited memory and only used a Word (axis) of MY PERSONAL BAG of English words.
I cant say wether or not its the right word, no actually im somwat shure its the wrong word.

However i remeber approximatly a position:
Read agian:

Its also cause i found a Study lately that found out that Cannabis detects the Daylenght somewhere in the Axis (in lack of better memory i call it Axis) . It was such a hard Term to remeber, but i am firm it was somewher were the branches split in differnt directions, just the Axis, OR it was where the Leaves branch away from the Stems. One of the other.
But they said that cannbis detects DEFINITLY the light Cycles at those Axis.

:):cautious::rolleyes:
 

romanoweed

Well-known member
Other than that, i would think a Branch might be what i would try aswell, but what i would try first is a Leave. i mean multiple Leaves.

However , it could be that with a branch, the Florigen is consumed too much by the grafted Branch on its own. And they pointed out it works with a Leave (on other species)... I would try a Leave , first.
 

Piff_cat

Well-known member
Another excerpt (the original article is closed but a copy exists with no pics..) :

This is about How to harvest Neviles Haze or other super long flowering Sativas in early September. Many many months ago while reading about flowering I happened upon a Mikhail Chailakhyan paper. He was the one that coined the term Florigen.
http://en.wikipedia.org/wiki/Florigen
I was working with grafting at the time and when I read his paper it floored me. The paper concerns longer flowering tobacco being flowered early by grafting with an early tobacco. The early tobacco was similar to autoflowers in weed. BINGO. Large lights flash in head. So I immediately plant some Tom Hills haze. My plan, graft a autoflower to a cloned THH and see if it flowers early. This rocked on for quite a while. My clones kept getting root rot and dying. The third set I got some to take. After letting them root good I grafted a NYLD auto to the THH with an approach graft. Basically skin away both plants and then tape together. Pics.
The small one on the top is THH.
105.jpg


close up
106.jpg


I was going to keep this to myself and do some breeding with Hazes. The decrease in flowering time would be a great boon to breeding. Unfortunately a tornado came, cops got everything, busted. Since I'm certainly not going to be growing any time soon I figured I'd tell everyone.

After I had started this I found someone in Russia was doing the same thing and had been successful with an autoflower grafted to a Kali Mist. "In Russia". You know they don't have the weather for Kali Mist there but he made it happen.

It's interesting but known that certain new technologys people seem to start doing them at the same time. Very odd but it happens quite frequently. I don't claim to be the first just that I had the same notion.

I hope someone will try this this year. Here's the results I know so far. The auto does not have to be as big as the 12/12 plant. It needs to be after the solstice before the 12/12 is grafted to the autoflower. It would be great to have a Nevilles Haze finish in Canada.
thie ie incredible info ! im trying this for sure thanks for cool info. i agree viva haze and sativa revolution!
 

romanoweed

Well-known member
An Exerpt of this article says that Lightcondition /Temperatures and so forth can counteract these Floewring-inducing Levels.. if i understand right. And if i understand right, that ma mean if i graft a bigger Auto branch onto plant i may have stronger influence on these levels to withstand and winn the upper hand so to speak?

read:
Are these characteristics of the SFT/SP ratio paradigm universal or unique to tomato? Accumulating observations indicate that growth and termination in reproductive and vegetative meristems in other plants are also dictated by the ratio of SFT and SP homologs. In Arabidopsis, termination of the inflorescence by tfl1 is associated with simple, smaller, and more oblique leaves in long days with photoperiodic induction of FT (i.e., high FT/TFL1 ratio); all of these characteristics can be reversed when FT levels are reduced by short days, low light, cold temperatures, or their combinations
 

Cerathule

Well-known member
"The molecular nature of the systemic floral signal, florigen, is a protein product encoded by the FT gene, which is highly conserved across flowering plants. FT is expressed in leaves and transported to the SAM. In SAM cells, FT interacts with its receptor 1433 in the cytoplasm and is translocated into the nucleus to interact with the transcription factor FD. The resultant triprotein complex, FAC, activates downstream genes to initiate floral transition in the SAM. FT distribution in the SAM explains its function during inflorescence development, and its contribution to the yield of some crops, through the regulation of balance between inflorescence branching and floral meristem formation"
 

Attachments

  • Molecular function of florigen.pdf
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