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Cloning

Snype

Active member
Veteran
Is it true that cloning off the same plant over and over again will week'in the potency ?

No it's not true. How do you think all these clone only OG's are still around from the 90's. Just keep the Moms healthy and there's no problems.
 

420somewhere

Hi ho here we go
Veteran
No debate it's called genetics ..

No debate it's called genetics ..

And the debate begins. ....again

I think it is mostly poor growers or people who's tolerance has hit the ceiling have a hard time excepting genetics.

I say if you've got the fire..rock it :party:

The first cut is as good as the last cut.
 

waveguide

Active member
Veteran
here's a dumb, semi-on topic question to co-opt the thread..

what are you using for rooting hormone nowadays? i've been taking clones for a quarter century and since i got bontone rooting powder i've __totally__ sucked at cloning, and that crap is all i can find around here now.
 

Hold Your Fire

Finding my way back home
Veteran
what are you using for rooting hormone nowadays? i've been taking clones for a quarter century and since i got bontone rooting powder i've __totally__ sucked at cloning, and that crap is all i can find around here now.

Been using Clonex gel for years now.

I get great results.
 

bucketswithsoil

support your local surfboard builder...
And the debate begins. ....again
...:smoker::lurk:

I think it is mostly poor growers or people who's tolerance has hit the ceiling have a hard time excepting genetics.

I say if you've got the fire..rock it :party:

The first cut is as good as the last cut.
..you gots fire..FLOW WITH IT,SIMPLE...been running same plants for almost 4years now,just keep pulling clones,vegg,FLW...repeat...i dont have "one certain mom" per say...

Been using Clonex gel for years now.

I get great results.
...
Right there with ya....thats basically the only NON organic matter in my stable,....well that and some maxiCrop ill use every now and then....clonex is good shit in my eyes....even thou i think i heard/read somewheres...that aloe might work just as well...i cant quite remember so dont hold me 100%...:plant grow:
 

EclipseFour20

aka "Doc"
Veteran
IMHO...two issues are at play.

1. Does a plant's DNA change over time? Answer is normally "no".
2. Can the environment effect a plant's DNA? Yes (DNA mutation...very rare) and through epigenetic changes (alteration the physical structure of DNA without changing/mutating the DNA).

"One example of an epigenetic change is DNA methylation — the addition of a methyl group, or a "chemical cap," to part of the DNA molecule, which prevents certain genes from being expressed. In many eukaryotes, including plants, DNA methylation provides a heritable mark that guides formation of transcriptionally silent heterochromatin. In plants, aberrant RNA signals direct DNA methylation to target sequences, sometimes appropriately and sometimes inappropriately. This chapter discusses the generation of RNA signals for epigenetic changes, the factors that mediate those changes, and some of the consequences of those changes for plant gene expression and genome integrity."

Source: http://www.ncbi.nlm.nih.gov/pubmed/15725056

And this article:

"Scientists at the Salk Institute for Biological Studies have identified patterns of epigenomic diversity that not only allow plants to adapt to various environments, but could also benefit crop production and the study of human diseases.

Published March 6 in Nature, the findings show that in addition to genetic diversity found in plants throughout the world, their epigenomic makeup is as varied as the environments in which they are found. Epigenomics is the study of the pattern of chemical markers that serve as a regulatory layer on top of the DNA sequence. Depending on where they grow, the plants' epigenomic differences may allow them to rapidly adapt to their environments.

Epigenomic modifications alter gene expression without changing the letters of the DNA alphabet (A-T-C-G), providing cells with an additional tool to fine-tune how genes control the cellular machinery. These changes occur not only in plants, but in humans as well.

"We looked at plants collected from around the world and found that their epigenomes are surprisingly different," says senior author Joseph R. Ecker, a professor in Salk's Plant Biology Laboratory and holder of the Salk International Council Chair in Genetics. "This additional diversity may create a way for plants to rapidly adapt to diverse environments without any genetic change in their DNA, which takes a very long time."

By understanding epigenomic alterations in plants, scientists may be able to manipulate them for various purposes, including biofuels and creating crops that can withstand stressful events such as drought. That knowledge of epigenomic changes in crop plants could tell producers what to breed for and could have a huge impact on identifying plants that can survive certain conditions and adapt to environmental stressors, says Ecker, who is also a Howard Hughes Medical Institute and Gordon and Betty Moore Foundation Investigator. "

Source: http://phys.org/news/2013-03-epigenetics-unveils-environments-world.html


Research the terms: epigenetic "DNA methylation"

This pdf titled, "Transgenerational epigenetic inheritance in plants" is very informative--www.researchgate.net/profile/Marie-Theres_Hauser/publication/51072470_Transgenerational_epigenetic_inheritance_in_plants/links/00b7d531a204ddba62000000.pdf

Things are not what they seem to be.
 
And the debate begins. ....again

There is no debate. Just people who know the science and people who don't. Bro science is not science.

Ever eaten a Red Delicious or Fuji apple? Those are called 'cultivars'; they are cuttings of cuttings of cuttings that have taken place over many decades. There is no genetic deterioration. Mainstream botany isn't at all confused about this topic; just 'experts' on cannabis forums.

It has been speculated that plants that undergo severe stress and ill-health for a long period of time might experience some degree of telemere erosion, with the genetic damage coming along with any cuttings taken. But as far as healthy cannabis plants go, there is no degradation.
 

DemonTrich

Active member
Veteran
no moms here, clone after clone after clone after clone. I don't have the space for 4-6 strains worth of moms.
 

stoned-trout

if it smells like fish
Veteran
not if done right....I don't like to keep the same exact mom for more than a year max 6 months average I would guess... once it gets all bonsai like and has been rootpruned a few times its ready to get replaced..i tend to start getting thinner less aggressive growing growth..i like big cuttings for clones so I replace mom
 

Wendull C.

Active member
Veteran
I have cloned the same OG going on 15 years and it is just as good and vigorous as the day I got it.
Other things can come into play to make deleterious traits but that is bad gardening not genetic drift.
I do however as trout said restart my moms every 6 months or so.
 

420somewhere

Hi ho here we go
Veteran
That's generally what I do ...

That's generally what I do ...

no moms here, clone after clone after clone after clone. I don't have the space for 4-6 strains worth of moms.

I had a Pre-98 Bubba going for years. When the Mom got tired, we took a new Mom from the clones.

:party:
 
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