Cloning problems... again

[Shliite]

My last unsuccessful attempt to take clones was with rockwool cubes. They werent treated correctly and there were some over watering issues as well.

I took the advice I got in my last thread and built a bubbler cloner. These cuttings are about 9 days old. The leaves are beginning to yellow and, despite no visible roots on 5 out of 6, some have actually produced some vegetative growth underwater.

There is a bit of a slimy/fuzzy looking stuff attached to the stems of the clones. Only one has produced a visible root. The air stone i used for the fairly small tupperware container may have been producing too large of bubbles and could be fairly ineffective at distributing bubbles to all 6 clones. I see this as a possible reason of one successfully rooting and the other 5 not.

Solution is currently:
~ half gallon distilled water
3 drops h202
3 drops superthrive
bag on top for humidity with ventilation holes

Procedure followed:
-Dipped in "dip n' grow liquid rooting concentrate" after cut at 45 degrees with sterile razor.

-Temps have ranged from 70 - low 90s when it has been hot.
-Water temps have probably been in the range of 68-80

* I should note that for their first 4 days they were in the same solution but without the h202 but 40ppms of PBP grow.
* They were also taken out of water temporarily (maybe 5-10 seconds tops) a couple times to be moved for water changes and to take a peek at them.

Im wondering if I should:
-Cut again above the fungus/bacteria/whatever is on the stem as if I were re-starting the process again - and if so use dip n' grow solution?
-Try scraping it off and change the water?
-Cool the water off a little bit?
-Supplement with a 30-60 ppms of PBP grow?
-Cut off H202 use or add more?

What i've just done:
-Removed all 6 cuttings and put into cups of distilled water and 2 drops h202 (as seen in the pictures)
-Changed the water to a solution of:
*half gallon distilled water
*3 drops h202 (3% variety)
-Attempted to wipe off some of the bacteria
-Put them back in the system

Pictures:















Thanks for the help in advance. I really want to get this done right so i can get going into some bigger DWC buckets.

 

[macster]

Just leave them, They're taking a bit longer is all. Have you tried a root enzyme, Also adding a tiny bite of bloom formula was recommended to me and I get 100% success rate in about 7 days

 

[Shliite]

so- cutting the stem again above the rot wouldnt work? how did this happen???

 

[Goose Duff]

 

[Shliite]

Goose Duff Looks like you just left on too many leaves... there are not enough roots to uptake the water/nutes necessary to support the photosynthesis from that much greenery up top.. oh and the distilled water is probably the biggest part of the problem.. try just some simple tap water.. surely anything is better than distilled

whats wrong with distilled water? would it be beneficial to remove some leaves at this point? changing the water? thanks..

 

[Guest]

just recut the ones with rot

 

[Guest]

The healthier and more robust the cuts the better chance of rooting.removing leaves will slow the dehydration.I noticed the cuts were strait cut on a 45 degree angle.cutting directly through the node is not a good Idea. If you have a mother starting over may be faster.

 

[HeadyPete]

Also, the more woody stem you leave, the longer it will take, if at all. Try less stem and make sure it is the soft greeny fleshy part, above where it starts to toughen up.

I second the less leaves suggestion. Just leave the growing tip and next node, but trim the leaves down to half their size, just cut off half the leaf.

How much light are they under? If there is too much, the cut will die because it's metabolism is raised from increased light energy but there are no roots to collect water for the plant. It transpires and dries out. Mist with tap water for the first few days.

 

[Shliite]

thanks for the comments and suggestions everyone.

I think i may have cut too close to the nodes, and perhaps not a clean enough cut as i can remember struggling to get the razor to chop cleanly on some of them. Also, the PBP grow i added in the beginning + the warm temps and warm water probably caused some bacteria growth. In addition to all that, too much vegetation was left on as some of you have noted. I also looked at my rooting concentrate directions which said to hold under for only 3-5 seconds on the less-woody plants (such as cannabis), which i may have exceeded on some. Also, the airstone i've been using doesnt really stay at the bottom of the tub thus distributing bubbles unevenly and not to all the stems.

I think i pretty much botched this one in 10 different ways. Live and learn. The mother plant isnt in my possession, so i went ahead and re-cut 5 out of 6 clones and placed in tap water pH adjusted to about 5.8. I clipped all but 1 fan leaf on them and trimmed it down a bit.

Still not too sure why i was advised against using distilled water... if anyone could shed some light on that i'd appreciate it.



*btw they are under 2 cool blue daylight CFLs (32w i think) and 1 regular 48w CFL.

 

[HeadyPete]

I would unscrew the 48 watter until your clones show new growth, which means they have rooted.

Definitely adding those nutes to a warm rez is asking for trouble. Alot of organic nutes do not do well in a rez. No nutes for clones or seedlings until they start really growing!

 

[Albert Hofmann]

Couple suggestions:
1)prepare your mother for cloning by leeching N from yor plant.
2)after cutting at a 45 scrape the bottom 1" with a razor
3)get your temp variations to +/-5 degrees

 

[Goose Duff]

 

[ItsGrowTime]

I agree with an earlier poster that you are making it harder than you should.

My thoughts in no particular order:
Dump all those rooting solutions and just stick with plain old tap water.
No nutes in the water are ever needed or advised.
One small 23w CFL 2 feet away is enough light for a small batch.
Keep the clones on a normal lights on/off period of 18/6. Roots grow most during dark period.
No humidity dome/bag is needed with bubble cloners.
When cutting always cut a 45 degree angle with a razor and leave a node 1/4" to 1/2" above the cut. Strip all foliage except the top leaves and maybe a lower, if clones are big enough.
Get a bigger airstone to cover all or most of your stems. The more bubbles the better.
Get a small aquarium heater or heatmat and dial the water in to a constant 78-80 degrees.

Here's my tap water bubble cloner. Styrofoam floating on exposed water (I dont lightproof, its not needed). 12" airstones underneath and a small aquarium heater. I get 90% rooted within 10 days. Keep it simple.

 

[MynameStitch]

See the thing is using distilled water is having no nutrients; your clones have manily nitrogen issues but one of them has magniseum and this is because distilled has hardly nothing in it; just pure h20.

Everyone has given solid information in helping you out here..... one thing is very important like Pete said; woody stems take longer.

SO you want to get clones from the lower part of the plant lowest possible.

Alot of people take cuttings from lower parts that would only produce popcorn size buds that way the plant can put it's energy into growing bigger buds that can get more light.