Chemical process of curing

[Junkyard Frog]

I usually only lurk and learn in this section but I believe I can add value to your search.

Freeze drying may your best bet. I have tried the inexpensive method (dry ice) in small amounts with great results. A vacuum chamber and pump at low pressure placed inside a freezer (chamber only) could facilitate curing larger quantities, but I have not tested that option.

You dry the sample for few days then freeze and place it in the chamber or container with dry ice (sample goes above the ice and a hole should be in the top of the container). Continue to freeze with low vacuum running or co2 gently pulls the moisture out of the sample.

The vacuum method would work best at ideal temp + pressure.

 

[Only Ornamental]

Not trying to win a pissing contest here but...
Freeze drying is a very efficient, fast, and gentle method for drying stuff which greatly reduces any chemical or biological reaction during the process. Usually, the samples aren't pre-dried and that results in physical/structural changes of the sample. Unless you cure fist and then use freeze drying only to speed up the final drying step.
Usually, freeze drying is used to remove any residual water (moisture content below 5%) whereas cannabis should still contain a certain amount of residual moisture or it won't burn nicely. Freeze drying makes it hard to stop early enough without repeated thawing-weighing-refreezing cycles (which BTW will accelerate sample degradation and reduce shelf life). Please be advised that poor/unprofessional or overly long freeze drying will remove essential oils quite efficiently and rupture the trichome heads.
That said, freeze drying is exactly the opposite of curing and hence your worst bet .

If you still want to try freeze drying: Do not add dry ice INTO the vacuum chamber or it will break the vacuum by quickly evaporating and hence you'll be either freezing or drying/vacuuming but not both at the same time. Put the dry ice around a "vacuum vessel" and the ice will also last way longer and this time, there will be a real vacuum. Besides, the aim of freeze drying isn't to "pull the moisture out of the sample" with CO2 gas. The correct term here is "sublimation" and that works better the stronger the vacuum is (or the lower the pressure).

 

[coldcanna]

The Biochemistry of Chlorophyll Degradation in Senescing Leaves


Protein mobilization in senescence is regulated by a network of processes (Dangl et al., 2000; Hörtensteiner and Feller, 2002; Thomas and Donnison, 2000), among which the induction of chlorophyll degradation is an early and, for plastid membrane polypeptides, essential event (Thomas et al., 2002). Net loss of chlorophyll from green tissues during senescence and other terminal developmental events culminates in the accumulation of colorless products (nonfluorescent chlorophyll catabolites, or NCCs) (Mühlecker and Kräutler, 1996). The enzymic pathway of NCC formation from chlorophyll (Hörtensteiner, 2004) commences with chlorophyllase, which dephytylates chlorophyll a. Magnesium (Mg) is removed from chlorophyllide a by a dechelatase activity. The tetrapyrrole macrocycle of the product of Mg removal is opened oxygenolytically by pheophorbide a oxygenase (PaO), producing a red bilin, RCC. A reductase immediately converts RCC into a colorless fluorescent product, FCC. Further enzymic and nonenzymic reactions metabolize FCC to NCCs in a species-specific manner (Hörtensteiner and Feller, 2002; Thomas et al., 2001). Catabolites of chlorophyll b are not normally observed in senescing tissues, leading to the notion that there is interconversion between chlorophyll(ide) a and b and catabolism exclusively by the a-specific pathway. An enzymic activity capable of converting chlorophyllide b to a has been shown to become elevated during senescence (Scheumann et al., 1999). Terminal catabolites are sequestered in the cell vacuole. There is no evidence that the N of the chlorophyll ring is exported from the cell during senescence. Chlorophyll catabolism is summarized in Fig. 1.


https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/chlorophyllase

 

[coldcanna]

Role of chlorophyllase in chlorophyll breakdown


Of high importance to all photosynthetic organisms is chlorophyll, and so, its synthesis and breakdown are closely regulated throughout the entire life cycle of the plant. Chlorophyll breakdown is most evident in seasonal changes as the plants lose their green color in the autumn; it is also evident in fruit ripening, leaf senescence and flowering. In this first step, chlorophyllase initiates the catabolism of chlorophyll to form chlorophyllide. Chlorophyll degradation occurs in the turnover of chlorophyll, as well as in the event of cell death caused by injuries, pathogenic attack, and other external factors.

Chlorophyllase’s role is two-fold as it functions in both de-greening processes, such as autumnal coloration, and is also thought to be involved in turnover and homeostasis of chlorophylls. Chlorophyllase catalysis of the initial step of chlorophyll breakdown is important for plant development and survival. The breakdown serves as a prerequisite in the detoxification of the potentially phototoxic chlorophyll and chlorophyll intermediates as it accompanies leaf senescence to non-fluorescent catabolites. Rapid degradation of chlorophyll and its intermediates is therefore necessary to prevent cell damage due to the potential phototoxicity of chlorophyll.[5][6][7]

Reaction and mechanism catalyzed by chlorophyllase
Chlorophyllase catalyzes the hydrolysis of ester bond to yield chlorophyllide and phytol. It reacts via tranesterification or hydrolysis of a carboxylic ester in which its natural substrates are 13-OH-chlorophyll a, bacteriochlorophyll and chlorophyll a.


Hydrolysis of chlorophyll starts with the attack of a carbonyl group of chlorophyll by the oxygen of the hydroxyl group of the crucial serine residue of the chlorophyllase. This attack forms a tetrahedral transition state. The double bond of the attacked carbonyl reforms and the serine is then esterified to chlorophyllide. The phytol group consequently leaves the compound and replaces the serine residue on the chlorophyllase enzyme. The addition of water to the reaction cleaves the phytol off the enzyme. Next, through the reverse reaction, the oxygen on the hydroxy group from the water in the previous step attacks the carbonyl of the intermediate in order to form another tetrahedral transition state. The double bond of the carbonyl forms again and the serine residue returns to chlorophyllase and the ester of the chlorophyll is now a carboxylic acid. This product is chlorophyllide.[8]

Chlorophyllide is then broken down to Pheophorbide A. After Pheophorbide a is formed, the poryphin ring is cleaved by Pheophorbide an oxide to form RCC causing the plant to lose its green color. RCC is then broken down into pFCC.

Regulation
Posttranslational Regulation
Citrus sinesis and Chenopodium album were the first plants from which the genes encoding chlorophyllase were isolated. These experiments revealed an uncharacteristic encoded sequence (21 amino acids in Citrus sinensis and 30 amino acids in Chenopodium album) located on the N-terminal that was absent from the mature protein. The chlorophyllase enzyme is a smart choice as the rate limiting enzyme of the catabolic pathway since degreening and the expression of chlorophyllase is induced in ethylene-treated Citrus. Recent data, however, suggests that chlorophyllase is expressed at low levels during natural fruit development, when chlorophyll catabolism usually takes place. Also, some data suggests that chlorophyllase activity is not consistent with degreening during natural senescence. Finally, there is evidence that chlorophyllase has been found in the inner envelope membrane of chloroplast where it does not come in contact with chlorophyll. Recent studies inspired by inconsistent data revealed that chlorophyllase in Citrus lacking the 21 amino sequence on the N-terminal results in extensive chlorophyll breakdown and the degreening effect that should occur in vivo. This cleavage occurs in the chloroplast membrane fraction. Both the full chlorophyllase and the cleaved, mature chlorophyllase, however, experienced similar levels of activity in an in vitro assay. This data suggests that the mature protein comes in contact with its substrate more readily because of the N-terminal sequence and some natural regulation occurs that directly affects enzyme activity. Another possibility is that the suborganelle compartments breaking down allowing a greater amount of enzyme activity.[9]

Other forms of regulation
Chlorophyllide, the product of the reaction catalyzed by chlorophyllase, spontaneously combines with plant lipids such as phosphatidylcholine liposomes along with sulfoquinovosyl diacylglycerol. These two lipids cooperatively inhibit the activity of chlorophyllase, but this inhibition can be reversed by the presence of Mg++, a divalent cation.[10] The activity of chlorophyllase also depends on the pH and ionic content of the medium. The values of kcat and kcat/Km of chlorophyllase in the presence of chlorophyll showed pKa values of 6.3 and 6.7, respectively. Temperature also affects chlorophyllase activity. Wheat chlorophyllase is active from 25 to 75 °C. The enzyme is inactivated at temperatures above 85 °C. Wheat chlorophyllase is stable 20 °C higher than other chlorophyllases. These other chlorophyllases can stay active at temperatures up to 55 °C.[11]

Ethylene induces the synthesis of chlorophyllase and promotes the degreening of citrus fruits. Chlorophyllase was detected in protein extracts of ethylene treated fruit. Ethylene treated fruits had chlorophyllase activity increased by 5 fold in 24 hours. Ethylene, more specifically, induces increased rates of transcription of the chlorophyllase gene.[12][13]

There is also evidence of a highly conserved serine lipase domain in the chlorophyllase enzyme that contains a serine residue that is essential for enzyme activity. Histidne and aspartic acid residues are also a part of the catalytic triad of chlorophyllase as a serine hydrolase. Specific inhibitors for the serine hydrolase mechanism, therefore, effectively inhibit the chlorophyllase enzyme. Also, mutations at these specific amino acid residues causes complete loss of function since the mutations change the catalytic site of the chlorophyllase enzyme.[8]

https://en.wikipedia.org/wiki/Chlorophyllase

 

[coldcanna]

Chlorophyllase Is a Rate-Limiting Enzyme in Chlorophyll Catabolism and Is Posttranslationally Regulated

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1867358/

 

[coldcanna]

Curing and subsequent aging allow for the slow oxidation and degradation of carotenoids in the tobacco leaf. This produces various compounds in the tobacco leaves that give cured tobacco its sweet hay, tea, rose oil, or fruity aromatic flavor that contributes to the "smoothness" of the consumed product. Non-aged or low quality tobacco is often artificially flavored with these otherwise naturally occurring compounds. Tobacco flavoring is a significant source of revenue for the international multimillion-dollar flavor and fragrance industry.[1]

The aging process continues for a period of months and often extends into the post-curing harvest process.


Carotenoids (/kəˈrɒtɪnɔɪd/), also called tetraterpenoids, are organic pigments that are produced by plants and algae, as well as several bacteria and fungi. Carotenoids can be produced from fats and other basic organic metabolic building blocks by all these organisms. The only animals known to produce carotenoids are aphids and spider mites, which acquired the ability and genes from fungi[1][2][3] or it is produced by endosymbiotic bacteria in whiteflies.[4] Carotenoids from the diet are stored in the fatty tissues of animals, and exclusively carnivorous animals obtain the compounds from animal fat.

There are over 600 known carotenoids; they are split into two classes, xanthophylls (which contain oxygen) and carotenes (which are purely hydrocarbons, and contain no oxygen). All are derivatives of tetraterpenes, meaning that they are produced from 8 isoprene molecules and contain 40 carbon atoms. In general, carotenoids absorb wavelengths ranging from 400–550 nanometers (violet to green light). This causes the compounds to be deeply colored yellow, orange, or red. Carotenoids are the dominant pigment in autumn leaf coloration of about 15-30% of tree species, but many plant colors, especially reds and purples, are due to other classes of chemicals.


Aroma chemicals

Products of carotenoid degradation such as ionones, damascones and damascenones are also important fragrance chemicals that are used extensively in the perfumes and fragrance industry. Both β-damascenone and β-ionone although low in concentration in rose distillates are the key odor-contributing compounds in flowers. In fact, the sweet floral smells present in black tea, aged tobacco, grape, and many fruits are due to the aromatic compounds resulting from carotenoid breakdown.

https://en.wikipedia.org/wiki/Carotenoid

 

[Only Ornamental]

Again: as long as the buds are still green there's considerable amounts of chlorophyll present (if not even most of it).
Me too, I was wondering if and/or to what extent carotenoid breakdown products contribute to the smell and aroma of cannabis. At least in tobacco high carotenoid content correlates with dark brown to red colours whereas progressive carotenoid degradation leads to paler shades like yellow and beige. These colours aren't as good an approximation of carotenoid content as green goes along with chlorophyll, though.

 

[coldcanna]

I guess a lot of it boils down to personal preference. The closest thing you are going to get to the original "flavor" of the living plant would be to freeze it fresh then vaporize it. Actually smoking cured flower seems like your going to get a much more degraded monoterpene effect with the carotenoids playing a stronger role in the experience.


Ornamental- from how you understand these enzymatic processes, are they oxygen dependent? One thought I has was co2 or nitrogen curing to slow the oxidization of monoterpenes down.

 

[Only Ornamental]

...
Ornamental- from how you understand these enzymatic processes, are they oxygen dependent? One thought I has was co2 or nitrogen curing to slow the oxidization of monoterpenes down.

Not all but many of these require oxygen. CO2 is not inert and will affect or even induce physical and chemical changes. Nitrogen seems perfect but without a bottle of nitrogen gas...
See also THIS thread.

 

[coldcanna]

Interesting read, I think our curiosity is in the same place. I could prob have access to nitrogen but my company doesn't have a GC-MS so unfortunately I can't give any proof on essential oils beyond my own senses.

 

[Only Ornamental]

Sometimes, your nose is better than a machine. Famous 'scent companies' such as Firmenich and Givaudan have a GC-Snif which is a common GC ending in a valve in front of a lab technicians nose and, well, that person acts as detector, sniffs what comes out and writes down how each peak smells . Peak intensity doesn't necessarily correlate with scent intensity and a machine can't tell flavours...

 

[Thcvhunter]

Asking and exploring these questionns will help you on your way:

Why cant a plant thats completely dry continue the curing process

What is actually being offgassed during curing.
What enzymes and bacteria do these processes.

Who does organic pot cure "better" and stay fresher for longer ;-)

How have tribes cured their pot asrobically.
How have tribes cured their pot anaerobically.

With the monetizing of pot, and more importantly, the monetizing of processes, I dont give people the answers but still gladly lead them along their journey.

Also, senescence and curing are different things/processes, though somewhat related.

 

[G.O. Joe]

Would anyone buy yellow much less brown pot since 1984?

 

[Thcvhunter]

Would anyone buy yellow much less brown pot since 1984?

Absolutely. When done right, its the best, most powerful pot in the world.
Now, this is proper curing, not talking about moldy schwag

 

[coldcanna]

Would anyone buy yellow much less brown pot since 1984?

A green bud indicates there is still plenty of chlorophyll. Think of leaves in autumn. When the caretenoids have fully degraded they turn yellow/brown. For that matter, fully cured tobacco is a golden brown color.

To your point, the science doesn't mean people will buy it because of the status quo being in their head....

 

[Betterhaff]

I remember a batch of Mexican we got in the mid seventies that was golden, the whole lot. Not a single other color, no browns, no reds, no purples, no green, just bright gold. Almost looked fake but was very, very good. Supposedly it was Acapulco Gold but probably from Oaxaca (marketing).

Carotinoids are responsible for the yellow and orange colors present but are masked by chlorophyll, as chlorophyll degrades, carotinoids display (referencing color change in leaves). Anthocyanins, which are the reds, blues, and purples are a different story.

 

[Only Ornamental]

Don't forget common flavonoids which are often present at higher concentrations than carotenoids and are usually of a whitish, yellow (hence their name) or orange colour whereas carotenoids may also be red. BTW anthocyanidins are a sub-class of flavonoids.

 

[Betterhaff]

That is correct, anthocyanins are a class of flavinoids, although anthocyanins continue or tend to be produced during senescence as opposed to carotinoids that are already there and not being produced anymore.

 

[coldcanna]

Correct me if I'm wrong but being that there are not many red, blues, purples in cannabis wouldnt that indicate a low amount of anthocyanins ?

 

[Betterhaff]

Probably more so in WLD types. Mustafunk just posted these in a landrace indica thread. Check out the images.

Potent, landrace and commercial don't mix well in the same sentence. Expect to spend a few years refining any landarce before doing any serious breeding with it. Otherwise, try to find maybe some Deep Chunk, X18 or something like that and you have most of the work done already by others. They are great representations of classic hashplants.


I usually avoid dramas but people needs to know what's up. USC went down the road after JGL retired from growing/breeding/smoking and left the company... Cristalin took over the company, but now they are mostly selling the genetics JGL creaed plus some stolen works from other people without their blessings.

Black Afghani came from a group of Russian landrace enthusiasts who used to hang around the Vibes Collective and Cannabiogen's forum time ago. The russians used sell their Black Kandahar and some other seeds at their own forum.

Since the first minute I've seen USC's pictures I knew I've seen that line before. I guess after so many years meeting people and discovering lines within the landrace/breeding scene it really pays off because to be honest, rare lines don't appear suddenly from nothing. Often they have been documented before.

Pics Cristalin uploaded:

View Image

Original pictures by Astronet and the Russian crew, the original name is Black Kandahar, not Black Afghani:

View Image

Pics from a newer thread where Siamon speaks about the Black Kandahar from The Russian Crew on Cannabiogen's forum and shows some images:

https://www.cannabiogen.com/club/viewtopic.php?f=34&t=4455&sid=be0c5c43ce4fa893cc01e79157e2b860



Then that Uzbekistan Pink Malabar is simply the so called Uzbeka line that Alex La Mano Negra from Spain used to have, Cristalin obtained the line after another Spanish member did a reproduction back then at the Vibes Collective and he's now just selling all those like if he was the one who spent years working on those. Anyway I don't think it's a real Uzbeki though, unfortunately LMN has always been quite secretive with his recipes and loved good histories, so often he would "exaggerate" lineages. But the truth and what really matters is that LMN was a talented breeder/grower and he never wanted his lines to be sold and exploited commercially, that's why he was giving so many seeds out, too bad he's no longer between us to stand up... such a con move IMO and as far as I know there are currently some others cashing on his genetics too .

So sad about USC, I used to have a great relationship with them... everything went down the road since their partnership with Tropical Seed Company cons, then all this. JGL worked his ass off to build up his reputation on the scene and in order to put his works and also USC on the map and now Cristalin is ruining everything because of his desperation to sell seeds and make some money from Cannabis, even if they aren't his own creations. I told JGL to be careful with his decissions and at the end he gave me the reason, it was just too much for him and gave up on the industry.

We need more humble and honest people on the scene, not fake breeders cashing on other people's legacy and doing anything for the money. We shouldn't be supporting and contributing to this kind of practices... so tired of watching other people cashing on other's legacy with absolute disrespect and no consequences. There is always a good option or better way to do things. Customer should be more responsible as well but many people only wants the product, they don't care what's behind it.

Funny thing is that then when you keep your stuff close to your chest to avoid this kind of stuff then you are called a hoarder, yet when you are kind, trustful and generous and give genetics out so others can enjoy too you are just simply stabbed in your back as the minimum chance of making some easy money appears. And then we wonder how often the most talented breeders or growers just dissapear from the scene, tired or dissapointed after having a dream on getting a living with something they used to love.



Damn...

https://www.icmag.com/ic/showthread.php?t=350365