It's been a while since I started a thread so I figure I'd reset the clock and start one.
I was bongin out thinking about plants that either produce very little viable seed when selfed or refuse to make any at all.
Exodus cheese and OGKB are two that readily come to mind but I'm sure there are more.
I was originally thinking about how sometimes when you reverse a plant the pollen clumps up and isn't readily carried off by the wind. I then got to thinking(keep in mind I'm not a scholar, I'm just a fkn stoner) that the plants were either producing something that was making the pollen stick together or failing to produce a substance that under normal circumstances would coat healthy pollen and not allow it to clump. I didn't and still don't know much about pollen so please go easy on me!haha
I was going on logic at this point so it was time to hit the books! Logic has screwed me before!lol I have a decent library so it didn't take long to find out that there is indeed a substance that coats each individual grain pollen. I forget the name. I'll try to find it and add later.
I wasn't able to find out if that substance kept the pollen grains from clumping up together but I did find out that the coating is what initiates fertilization by growing a tube(pollen tube) that eventually penetrates the ovule so the pollen grain can transfer its DNA into the egg.(something like that)haha
That finally got me to thinking...... what if the plants that won't self fertilize are like that because when they're reversed, the plant produces pollen without the coating or the coating isn't produced correctly, not allowing the pollen tube to grow. Again... I'm using stoner logic so be nice!haha
The thing I had to get past was obvious, how to get the stuff off of the good pollen(pollen that will fertilize OGKB/Exodus) and get it on the reversed OGKB/Ezodus pollen in the hopes that it would improve fertilization without the "donor" pollen fertilizing anything.
Well.....I don't post much about what I'm into because I'm into things that can be controversial and a liability to talk about. The breeding techniques I've been interested in for nearly 20 years can be dangerous for the experienced, nevermind the inexperienced. I'm not gonna go to far into it but it it did give me an idea.
I could sterilized the pollen with X-rays(I have very powerful X-ray generators). I could hit them with extreme UV rays(modified 1000mh bulb) I could even expose them to all kinds of radioactive material.haha
Anyone of these treatments would sterilize the pollen without affecting the "coating". The sterile pollen could then be placed in the same vial as the OGKB/Exodus pollen and mixed. Theoretically coating the pollen without the substance with the substance from the donor pollen.
Because this substance will still be on the donor (sterilized)pollen it will help grow the pollen tube for the pollen that wasn't coated as well.
The important thing would be to make damn sure that the donor pollen is completely sterilized so that there won't be any unwanted fertilization. Anything short of complete sterilization using these techniques would more than likely lead to extreme mutations in any of the seeds that made it plus they'd be fathered by the wrong plant.
EXTREME CARE would have to be taken of course.
This is just a theory as I haven't been able to source either cuts. Truth be told, I haven't really tried all that hard to get them.
If OGKB phenos are polyploid(I haven't been able to verify this either) than this won't help as chromosome incompatibility is a different and known subject.
I'm not sure about any of this as it's only a theory. I'm interested in any thoughts though.
I have other thoughts on how this technique could possibly be used to do other things but ethics prevent me from going into this further.haha
I was bongin out thinking about plants that either produce very little viable seed when selfed or refuse to make any at all.
Exodus cheese and OGKB are two that readily come to mind but I'm sure there are more.
I was originally thinking about how sometimes when you reverse a plant the pollen clumps up and isn't readily carried off by the wind. I then got to thinking(keep in mind I'm not a scholar, I'm just a fkn stoner) that the plants were either producing something that was making the pollen stick together or failing to produce a substance that under normal circumstances would coat healthy pollen and not allow it to clump. I didn't and still don't know much about pollen so please go easy on me!haha
I was going on logic at this point so it was time to hit the books! Logic has screwed me before!lol I have a decent library so it didn't take long to find out that there is indeed a substance that coats each individual grain pollen. I forget the name. I'll try to find it and add later.
I wasn't able to find out if that substance kept the pollen grains from clumping up together but I did find out that the coating is what initiates fertilization by growing a tube(pollen tube) that eventually penetrates the ovule so the pollen grain can transfer its DNA into the egg.(something like that)haha
That finally got me to thinking...... what if the plants that won't self fertilize are like that because when they're reversed, the plant produces pollen without the coating or the coating isn't produced correctly, not allowing the pollen tube to grow. Again... I'm using stoner logic so be nice!haha
The thing I had to get past was obvious, how to get the stuff off of the good pollen(pollen that will fertilize OGKB/Exodus) and get it on the reversed OGKB/Ezodus pollen in the hopes that it would improve fertilization without the "donor" pollen fertilizing anything.
Well.....I don't post much about what I'm into because I'm into things that can be controversial and a liability to talk about. The breeding techniques I've been interested in for nearly 20 years can be dangerous for the experienced, nevermind the inexperienced. I'm not gonna go to far into it but it it did give me an idea.
I could sterilized the pollen with X-rays(I have very powerful X-ray generators). I could hit them with extreme UV rays(modified 1000mh bulb) I could even expose them to all kinds of radioactive material.haha
Anyone of these treatments would sterilize the pollen without affecting the "coating". The sterile pollen could then be placed in the same vial as the OGKB/Exodus pollen and mixed. Theoretically coating the pollen without the substance with the substance from the donor pollen.
Because this substance will still be on the donor (sterilized)pollen it will help grow the pollen tube for the pollen that wasn't coated as well.
The important thing would be to make damn sure that the donor pollen is completely sterilized so that there won't be any unwanted fertilization. Anything short of complete sterilization using these techniques would more than likely lead to extreme mutations in any of the seeds that made it plus they'd be fathered by the wrong plant.
EXTREME CARE would have to be taken of course.
This is just a theory as I haven't been able to source either cuts. Truth be told, I haven't really tried all that hard to get them.
If OGKB phenos are polyploid(I haven't been able to verify this either) than this won't help as chromosome incompatibility is a different and known subject.
I'm not sure about any of this as it's only a theory. I'm interested in any thoughts though.
I have other thoughts on how this technique could possibly be used to do other things but ethics prevent me from going into this further.haha
