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Photoperiod manipulation for Pheno manipulation

Elevator Man

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I've been intrigued for some time by DJ Short's assertions regarding photoperiod and environment having more influence on the phenotype of a plant than most other stimuli. I was particularly taken by the premise that the 18/6 and 12/12 lighting regimes would generally tilt a 50/50 hybrid towards the indica, in terms of effects, at least. His thesis holds that the closer to an equatorial photoperiod a plant can be kept, the more likely that it will express the sativa side of its nature.

So I decided to test the theory, and try and find out a little about what's inside some of my hybrid mothers, in terms of indica/sativa balance, and whether it could be altered. I've long wondered whether a different veg period to the 18-hour standard was key to the puzzle, so I decided to try a little experiment.
I wanted to find the ideal veg period for a plant that would allow it to flower quicker, and flower more profusely. Also I wanted to find out whether changing the photoperiod would throw up any anomalies in terms of stability, etc. And of course, whether there could be a better 'standard' than 18/6 that would allow the sativa side to be enhanced - which I prefer. I'm not a couchlock kind of guy...:)

So - over a period of two months, I very gradually reduced my veg light period, from 18 hours to 15. I left the plants on 15/9 for one month, and observed the results. All but one plant showed signs of budding, some more than others. As expected, most of the 'indica-weighted' hybrids started budding profusely. Most of the sativa-weighted plants were more hesitant, presumably as 15 hours is not short enough to induce flowering.*

After one month, it was obvious that some plants were sliding into proper flowering mode, and so I gradually upped the photperiod again, stopping at 16 hours, where it has remained for the last three months. During that time several significant changes have happened.

The most 'indica' plant I have (by this criterion, anyway) was a Nirvana NL X BB, and this took literally months to cease flowering and begin re-vegging (by re-vegging, I mean new single-blade leaves appearing and new shoots following). Others with more sativa dominance re-vegged extremely quickly, and resumed normal veg growth.

However, the one plant I have long suspected to be NL#5, which is 25% Thai, has lost it's indica appearance, and has started growing like a Thai - much longer, fluffier buds and many more shoots with a less regular structure than before. This appears to confirm DJ Short's theory that a more sativa pheno can be 'coaxed' out of a mixed or indica-dominant pheno.

Another major discovery for me, confirmed so far on subsequent grows, is that the plants now flower far quicker than before when switched to 12/12. It used to be 10-14 days for real buds to appear - now it is 7-10. Again, all good.

The one plant that has so far resisted all photoperiod changes is my 'G13' - in quotes as I have no way of ascertaining it in reality - suffice to say it has very droopy, long-bladed leaves, and looks very indica, but it is normally somewhat sativa in growth pattern - a bit random, with weaker stems than most indicas.

My Jack Herer was binned as the final step of this experiment - it was never happy being tweaked like this, and went so out of control (in a sativa kind of way) that it was losing any sense of what it was - hard to describe, but it was as though the pheno were 'breaking apart' into 'separate personalities' (like in a horror movie!). The new, more ruthless gardener now inside me decided that this was too runty for my growroom, so I chopped it. Resistance to extreme photoperiods was another trait I wanted to check for, and it sadly failed the test...:)

I have no pics of any of these plants yet, but I will post some when I grow out all my mothers in a couple of months.

So - just a quick rundown of what happened to each:

Northern Lights X Big Bud (previously indica-dominant) - still pretty much indica-dominant, with great resistance to change - starts flowering at 15 hours, and has hardly stopped flowering at 16 hours (I'm still cutting min-buds off). 3 months to even begin re-vegging. However, rock-solid and very alive.

'G13' (indica leaf pattern, sativa brancing) - has hardly been affected by any of the experiment - refused to flower at 15 hours, and happily went back into veg at 16 hours. Barely no pre-flowers or pre-buds, unlike every other plant. Happy as Larry.

Crisscross (sativa-dominant, fast-flowering hybrid - the 'local C99' I like to think of it - not that I've ever tried C99!) - resisted 15 hours quite well, with very small buds appearing. Re-vegged very quickly at 16 hours, so confirming the hyrbid to be sativa-dominant.

Jack Herer (sativa-dominant pheno) - this strain just seemed to get 'confused' by the whole experiment, with many shade leaves appearing to wither as though flowering was complete, but with no flowering. Weird buds formed, but many indica-type mixed with sativa-type. Switching back to 16 hours just seemed to make it worse - by the end, it resembled DJ's desciption of the Juicy Fruit Thai, but very ill. So I binned it...sorry, Sensi...:)

Mystery mother (suspected to be NL#5) - this fared well, and began budding in sync with the NL X BB. The main difference was that the plant stopped flowering much more quickly when returned to 16 hours, and has adjusted it's branching and bud structure to appear much more sativa now.

The two Flos were also the first to be fully flowered from the new regime, and went from 16/8 to one week of 13/11, six weeks at 12/12 and one week at 11/13. However, as they didn't exist before it wasn't fair to include them. But they did bud in 7 days...:)

So the next grow will test all the non-pheno aspects of the theory - i.e. do they SMOKE more like sativas now? Of course, I will post the results in due course.

But as to the main premise that a 16/8, or other non-standard veg period may be more beneficial for 'bringing out' sativa traits in a balanced hybrid - does anyone have similar experiences or agree/disagree with the premise itself? I'd love to get some feedback.

Many thanks.


* As an aside to seed breeders, this would be VERY useful data when buying seeds - if the minimum trigger point for each strain could be published on the packet, along with the flowering period, it would make things a lot easier for issues such as this! Is that possible? :)
 
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iamhigh

Member
just letting you know you just blew my mind... :yoinks: great stuff.. hope someone can post with any expirence on this subject cause id love to know more... :woohoo:
 

TUTIDO

Member
That's some really interesting work you're doing there, definately can't wait to hear more on the subject.
 
V

vhGhost

Elevator Man , first of - thank you... your doing all the hard work that some just don't have the time or the room to try. i've also been reading a thread here with some info on flowering with only 6hours(on) and 12hours(off).... but again great info and please keep us updated.

ps. i run the 24/7 beg period and i've noticed that if i take my plants and go from 24/7 to 18/6 before 12/12 for about 1 week that they bud alot faster... ( plants think winter is coming ? ) ...
 

Teadaemon

Member
It's a fascinating idea, but really to get any kind of meaningful data you'd need several very large grows - I'd suggest at least 100 plants of each variety, preferable 10 times that. I'd also suggest that a serious experiment would need a similarly sized control group whose photoperiod wasn't mucked around with. I also think that differences (time for flowers to appear at different photoperiods, leaf shape, internode length, location of bud sites, etc) need to be better quantified, rather than a subjective assesment of sative/indica proportions.

I don't want to sound too negative, 'cos this is quite an interesting idea that may have something behind it, but at the same time I wouldn't want anyone to draw firm conclusions from a very limited, uncontrolled experiment.
 

Guest423

Active member
Veteran
Teadaemon said:
It's a fascinating idea, but really to get any kind of meaningful data you'd need several very large grows - I'd suggest at least 100 plants of each variety, preferable 10 times that. I'd also suggest that a serious experiment would need a similarly sized control group whose photoperiod wasn't mucked around with. I also think that differences (time for flowers to appear at different photoperiods, leaf shape, internode length, location of bud sites, etc) need to be better quantified, rather than a subjective assesment of sative/indica proportions.

I don't want to sound too negative, 'cos this is quite an interesting idea that may have something behind it, but at the same time I wouldn't want anyone to draw firm conclusions from a very limited, uncontrolled experiment.


maybe DJ has done that, and now elevator man is just experimenting to see if it holds true on a smaller scale, either way experimenting is a good thing....finding out the exact light cycle a strain starts to bud at is perfect for outdoor planning! keep it up, i have NL x BB and with the cycle you say it flips into budding is the exact cycle i would want mine to start flowering outdoors!
 

Teadaemon

Member
Time2Unite said:
maybe DJ has done that, and now elevator man is just experimenting to see if it holds true on a smaller scale, either way experimenting is a good thing....finding out the exact light cycle a strain starts to bud at is perfect for outdoor planning! keep it up, i have NL x BB and with the cycle you say it flips into budding is the exact cycle i would want mine to start flowering outdoors!

Fair enough, to a point. I just think it's worth pointing out that by experimenting on such a small scale, with such a wide range of variables, the chances of being able to pull any kind of meaningful data out is close to zero.

Sorry, it's the pedantic scientist part of my personality showing through, I'd better go toke up and chill out. :joint:
 

TML16

Snow Grower ~OGA~
Veteran
brilliant experiment and great share of a researched project.

I've always been a fan of "you'll never know till you try yourself"

So the next grow will test all the non-pheno aspects of the theory - i.e. do they SMOKE more like sativas now? Of course, I will post the results in due course.
I'm looking very forward to your conclusion

I have been a 16/8 vegger for my last 2 grows now. I read on Dutch Passion's website that lesser hours of light, higher levels of humidity, and higher levels of N produce a better female/male ratio from seed. I put their theory to the test and have been very successfull in my female ratio.

I have never thought to notice any difference in the sat to indi traits though. My last grow, both plants did show good sativa traits...now you got me wondering if I was unknowingly encouraging this.

Thanks for your time Elevator Man very well done
 

Elevator Man

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I fully accept that there's no control here - I would love to run a parallel grow on the standard cycle and compare the two. However, my 'mother room' is little more than a cupboard, and so it's tricky to expand on this. I DO have much more room available, but I rent, and so I'm very wary of turning my entire space into a breeding laboratory, much as I would like to!
That said though, I do get the impression that DJ HAS done many experiments on this - he says as much in his book, and says he uses a drastically different photoperiod to the standard (which he does not reveal), but suggests some timings that might prove to work.

As far as 'hard work' goes, well, I figured if I'm keeping the mothers anyway, then I may as well play around with them a little. The way some strains can change their appearance over time still amazes me, and I suspect a lot of it is down to finding the right environment.
For example, one of the purple Flo cuts that I gave to a friend has been grown outdoors for the last 12 weeks - what was a delicate-looking thing has evolved into an absolute monster. The leaves are far more indica-shaped than mine, and it has reverted to a more columnar structure.
Given the sun we've had in the UK lately, I think the plant is really showing its full potential now, and it's a great relief to see a plant I thought had problems, explode so healthily. If he'll let me photograph his garden, I will definitely post some of those...:)
 

Elevator Man

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I should also mention that the other main piece of data that was crucial to my understanding of this possible manipulation, was the photoperiod graph in Rob Clarke's Marijuana Botany, and a sunrise/sunset chart (and Google Earth!). And it was so simple, that I'm amazed I didn't twig before.

Simply that, at around 60 degrees latitude (Northern Hemisphere), there's around an 18-hour light period on June 21st. At 40 degrees, that drops to 15 hours. At 20 degrees, it's only 13.5. So, if all the plants are on a veg cycle of 18 hours a day, they 'think' that they are in the Shetland Islands (for argument's sake) on the longest day of the year - always.
So I figured, if I reduce the day period to 16 hours, the plants will 'think' that they're in Spain, Northen Cali, etc. on June 21st. Moving them to 15 hours would be getting them 'nearer' to Morocco or Northern Mexico. I also figured therefore, that the more sativa a plant, the lower a day-period it could handle without flowering, as it would be much closer to it's 'natural' photoperiod. However, 15 hours seemed to be the threshold for the indica-dominant plants, and since I don't have spares of any of these, I didn't dare go any lower. I suspect the two sativa-dominant plants could have handled 14 hours without flowering.

But I definitely think that suggesting a trigger-time for each strain would be fantastic info for any grower when buying seeds. I might just start a small thread in the Breeder froum, just to keep this topic alive...:)
 

jaykush

dirty black hands
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now this is what you do after breeding a strain, to find out how your shit grows best. what it wants best, how it likes it best. good post man, props to you.
 

Elevator Man

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Fantastic stuff - I have some free time, so I'll peruse this now. Good to see others have already spent some time thinking about this one...:)
 

Elevator Man

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Wow - excellent. That is exactly what I was hoping you were going to write! Well, I feel a little more certain on this now, and I think there may be something to take forward.
And I am also fishing-obsessed, which is a rather strange coincidence, eh? :)
 

budchopper

Active member
TUTIDO said:
That's some really interesting work you're doing there, definately can't wait to hear more on the subject.
is making a sativa/indica cross more sativa worth it when you can just buy
strains with a higher sativa ratio?
 
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