Chimera,
If you test a female that has been STS treated to turn male, with a RAPD or other marker DNA test what sex does it show?
Same with a male plant treated with ethephon to turn female, test it and what sex does it show?
If you are testing with RAPD or other markers to find males do you also test with RAPD or other markers to confirm they are female? Have you ever done female RAPD or other DNA testing?
If you test a full blown inter-sexed plant what is the results? With which tests? Does it make a difference if you use plant DNA from the male or female parts of an inter-sex plant?
-SamS
For everyone else here are some references for DNA based sex determination:
If you know of more please post a reference so I can get them. I am looking for an early Chinese and even earlier Hungarian sexing via DNA article. Anything about MADC1 or MADC2 or MADC 3, MADC4, MADC5, MADC6, SCAR332 or any others.
Theoretical and Applied Genetics
January 1999, Volume 98, Issue 1, pp 86-92
Identification of DNA markers linked to the male sex in dioecious hemp (Cannabis sativa)
G. Mandolino, A. Carboni, S. Forapani, V. Faeti, P. Ranalli
A male-associated DNA sequence in a dioecious plant, Cannabis sativa L.
Sakamoto K, Shimomura K, Komeda Y, Kamada H, Satoh S.
Plant Cell Physiol. 1995 Dec;36(8):1549-54.
Oxford Journals Life Sciences Plant and Cell Physiology Volume 36, Issue 8Pp. 1549-1554.
1995, A Male-Associated DNA Sequence in a Dioecious Plant, Cannabis sativa L.
Koichi Sakamoto, Koichiro Shimomura, Yoshibumi Komeda, Hiroshi Kamada and Shinobu Satoh
Maybe this one:
Sakamoto K, Akiyama Y, Fukui K, Kamada H, Satoh S (1998) Characterization; genome sizes and morphology of sex chromosomes in hemp (Cannabis sativa L.). Cytologia 63: 459–464. doi: 10.1508/cytologia.63.459
Identification of DNA markers linked to the male sex in dioecious hemp (Cannabis sativa L.)
G. Mandolino, A. Carboni, S. Forapani, V. Faeti, P. Ranalli
Theoretical and Applied Genetics (Impact Factor: 3.66). 12/1998; 98(1):86-92.
Maybe this one:
Comparison of Hemp Varieties Using Random Amplified Polymorphic DNA Markers
Crop Science Vol 41 nov-Dec 2000 pg 1682-1689
Silvia Forapani, Andrea Carboni, Claudia Paoletti, V. M. Cristiana Moliterni, Paolo Ranalli,
and Giuseppe Mandolino
Maybe this one:
Sakamoto K, Ohmido N, Fukui K, Kamada H, Satoh S (2000) Site-specific accumulation of a LINE-like retrotransposon in a sex chromosome of the dioecious plant Cannabis sativa. Plant Mol Boil 44: 723–732.
Euphytica
07-2002, Volume 126, Issue 2, pp 211-218
Occurrence and frequency of putatively Y chromosome linked DNA markers in Cannabis sativa L.
Giuseppe Mandolino, Andrea Carboni, Manuela Bagatta, V.M. Cristiana Moliterni, Paolo Ranalli
Törjék O, Bucherna N, Kiss E, Homoki H, Finta-Korpelová Z, et al. (2002) Novel male-specific molecular markers (MADC5, MADC6) in hemp. Euphytica 127: 209–218. doi: 10.1023/a:1020204729122
Törjék O, Bucherna N, Kiss E, Homoki H, Finta-Korpelová Z, et al. (2002) Novel male-specific molecular markers (MADC5, MADC6) in hemp. Euphytica 127: 209–218.
Theoretical and Applied Genetics
June 2003, Volume 107, Issue 1, pp 102-109
Sex-linked AFLP markers indicate a pseudoautosomal region in hemp (Cannabis sativa L.)
A. Peil, H. Flachowsky, E. Schumann, W. E. Weber
Journal of Industrial Hemp Vol 8 Number 1, 2003, Female-Associated DNA Polymorphisms of Hemp (Cannabis sativa L.) Hong Shao, Shu-Juan Song, Robert C Clarke
Plant Breeding
Volume 124, Issue 2, pages 167–170, April 2005
Sex-linked SSR markers in hemp
J. Rode, K. In-Chol, B. Saal, H. Flachowsky, U. Kriese andW. E. Weber
Sakamoto K, Abe T, Matsuyama T, Yoshida S, Ohmido N, et al. (2005) RAPD markers encoding retrotransposable elements are linked to the male sex in Cannabis sativa L. Genome. 48: 931–936.
RAPD markers encoding retrotransposable elements are linked to the male sex in Cannabis sativa L.
Koichi Sakamoto, Tomoko Abe, Tomoki Matsuyama, Shigeo Yoshida, Nobuko Ohmido, Kiichi Fukui, Shinobu Satoh
Genome, 2005, 48(5): 931-936
Plant Biotechnology
Electronic Journal of Biotechnology ISSN: 0717-3458 Vol. 10 No. 4, Issue of October 15, 2007
DOI: 10.2225/vol10-issue4-fulltext-4
RESEARCH ARTICLE
Inter simple sequence repeats separate efficiently hemp from marijuana (Cannabis sativa L.)
Erdogan E. Hakki, Seyit A. Kayis, Emine Pinarkara, Ayla Sag
Maybe this one:
Yang MQ, van Velzen R, Bakker FT, Sattarian A, Li DZ, et al. (2013) Molecular phylogenetics and character evolution of Cannabaceae.Taxon. 62: 473–485.
Maybe this one:
Molecular Cytogenetic Characterization of the Dioecious Cannabis sativa with an XY Chromosome Sex Determination System
Mikhail G. Divashuk, Oleg S. Alexandrov, Olga V. Razumova, Ilya V. Kirov, Gennady I. Karlov
Published: January 21, 2014 at PLOS One
If you test a female that has been STS treated to turn male, with a RAPD or other marker DNA test what sex does it show?
Same with a male plant treated with ethephon to turn female, test it and what sex does it show?
If you are testing with RAPD or other markers to find males do you also test with RAPD or other markers to confirm they are female? Have you ever done female RAPD or other DNA testing?
If you test a full blown inter-sexed plant what is the results? With which tests? Does it make a difference if you use plant DNA from the male or female parts of an inter-sex plant?
-SamS
For everyone else here are some references for DNA based sex determination:
If you know of more please post a reference so I can get them. I am looking for an early Chinese and even earlier Hungarian sexing via DNA article. Anything about MADC1 or MADC2 or MADC 3, MADC4, MADC5, MADC6, SCAR332 or any others.
Theoretical and Applied Genetics
January 1999, Volume 98, Issue 1, pp 86-92
Identification of DNA markers linked to the male sex in dioecious hemp (Cannabis sativa)
G. Mandolino, A. Carboni, S. Forapani, V. Faeti, P. Ranalli
A male-associated DNA sequence in a dioecious plant, Cannabis sativa L.
Sakamoto K, Shimomura K, Komeda Y, Kamada H, Satoh S.
Plant Cell Physiol. 1995 Dec;36(8):1549-54.
Oxford Journals Life Sciences Plant and Cell Physiology Volume 36, Issue 8Pp. 1549-1554.
1995, A Male-Associated DNA Sequence in a Dioecious Plant, Cannabis sativa L.
Koichi Sakamoto, Koichiro Shimomura, Yoshibumi Komeda, Hiroshi Kamada and Shinobu Satoh
Maybe this one:
Sakamoto K, Akiyama Y, Fukui K, Kamada H, Satoh S (1998) Characterization; genome sizes and morphology of sex chromosomes in hemp (Cannabis sativa L.). Cytologia 63: 459–464. doi: 10.1508/cytologia.63.459
Identification of DNA markers linked to the male sex in dioecious hemp (Cannabis sativa L.)
G. Mandolino, A. Carboni, S. Forapani, V. Faeti, P. Ranalli
Theoretical and Applied Genetics (Impact Factor: 3.66). 12/1998; 98(1):86-92.
Maybe this one:
Comparison of Hemp Varieties Using Random Amplified Polymorphic DNA Markers
Crop Science Vol 41 nov-Dec 2000 pg 1682-1689
Silvia Forapani, Andrea Carboni, Claudia Paoletti, V. M. Cristiana Moliterni, Paolo Ranalli,
and Giuseppe Mandolino
Maybe this one:
Sakamoto K, Ohmido N, Fukui K, Kamada H, Satoh S (2000) Site-specific accumulation of a LINE-like retrotransposon in a sex chromosome of the dioecious plant Cannabis sativa. Plant Mol Boil 44: 723–732.
Euphytica
07-2002, Volume 126, Issue 2, pp 211-218
Occurrence and frequency of putatively Y chromosome linked DNA markers in Cannabis sativa L.
Giuseppe Mandolino, Andrea Carboni, Manuela Bagatta, V.M. Cristiana Moliterni, Paolo Ranalli
Törjék O, Bucherna N, Kiss E, Homoki H, Finta-Korpelová Z, et al. (2002) Novel male-specific molecular markers (MADC5, MADC6) in hemp. Euphytica 127: 209–218. doi: 10.1023/a:1020204729122
Törjék O, Bucherna N, Kiss E, Homoki H, Finta-Korpelová Z, et al. (2002) Novel male-specific molecular markers (MADC5, MADC6) in hemp. Euphytica 127: 209–218.
Theoretical and Applied Genetics
June 2003, Volume 107, Issue 1, pp 102-109
Sex-linked AFLP markers indicate a pseudoautosomal region in hemp (Cannabis sativa L.)
A. Peil, H. Flachowsky, E. Schumann, W. E. Weber
Journal of Industrial Hemp Vol 8 Number 1, 2003, Female-Associated DNA Polymorphisms of Hemp (Cannabis sativa L.) Hong Shao, Shu-Juan Song, Robert C Clarke
Plant Breeding
Volume 124, Issue 2, pages 167–170, April 2005
Sex-linked SSR markers in hemp
J. Rode, K. In-Chol, B. Saal, H. Flachowsky, U. Kriese andW. E. Weber
Sakamoto K, Abe T, Matsuyama T, Yoshida S, Ohmido N, et al. (2005) RAPD markers encoding retrotransposable elements are linked to the male sex in Cannabis sativa L. Genome. 48: 931–936.
RAPD markers encoding retrotransposable elements are linked to the male sex in Cannabis sativa L.
Koichi Sakamoto, Tomoko Abe, Tomoki Matsuyama, Shigeo Yoshida, Nobuko Ohmido, Kiichi Fukui, Shinobu Satoh
Genome, 2005, 48(5): 931-936
Plant Biotechnology
Electronic Journal of Biotechnology ISSN: 0717-3458 Vol. 10 No. 4, Issue of October 15, 2007
DOI: 10.2225/vol10-issue4-fulltext-4
RESEARCH ARTICLE
Inter simple sequence repeats separate efficiently hemp from marijuana (Cannabis sativa L.)
Erdogan E. Hakki, Seyit A. Kayis, Emine Pinarkara, Ayla Sag
Maybe this one:
Yang MQ, van Velzen R, Bakker FT, Sattarian A, Li DZ, et al. (2013) Molecular phylogenetics and character evolution of Cannabaceae.Taxon. 62: 473–485.
Maybe this one:
Molecular Cytogenetic Characterization of the Dioecious Cannabis sativa with an XY Chromosome Sex Determination System
Mikhail G. Divashuk, Oleg S. Alexandrov, Olga V. Razumova, Ilya V. Kirov, Gennady I. Karlov
Published: January 21, 2014 at PLOS One
Last edited: